Thermo Fisher Scientific GM-CSF Monoclonal Antibody (MP1-22E9), eBioscience
마우스 GM-CSF에 특이적인 중화 단일클론 항체로 다양한 면역분석(ELISA, WB, Flow 등)에 사용 가능. 높은 순도(>90%)와 낮은 응집도(<10%)를 보유하며, 액상 형태로 PBS 완충액에 보관. 연구용으로 GM-CSF 관련 세포 성장 및 분화 연구에 적합.
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Thermo Fisher Scientific GM-CSF Monoclonal Antibody (MP1-22E9), eBioscience
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | Assay-Dependent | 1 publication |
| Immunohistochemistry (Frozen) (IHC (F)) | Assay-Dependent | - |
| Immunocytochemistry (ICC/IF) | - | 1 publication |
| Flow Cytometry (Flow) | - | 12 publications |
| ELISA | 1–4 µg/mL | - |
| Neutralization | - | 6 publications |
| Functional Assay | - | 1 publication |
| Miscellaneous PubMed | - | 1 publication |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Mouse |
| Published Species | Mouse |
| Host / Isotype | Rat / IgG2a, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | MP1-22E9 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_468485 |
Additional Formats:
- APC (17-7331-82)
- FITC (11-7331-82)
- Functional Grade (16-7331-81)
- PE (12-7331-82)
- PE-Cyanine7 (25-7331-82)
- Custom conjugation available upon request
Product Specific Information
The MP1-22E9 antibody reacts with mouse granulocyte/macrophage colony-stimulating factor (GM-CSF) and functions as a neutralizing antibody. Mouse GM-CSF is a 14 kDa factor produced primarily by activated T cells and macrophages, but also by endothelial cells and fibroblasts in response to TNF-alpha, IL-2, IL-1, and IFN-gamma. GM-CSF promotes the growth of macrophages, granulocytes, and dendritic cells, and exists both as a membrane-bound form and as a complex with the extracellular matrix. Non-glycosylated GM-CSF is biologically active.
Applications Reported:
This antibody has been used for ELISA capture, intracellular staining, flow cytometry, neutralization assays, western blotting, and immunohistochemistry on frozen sections.
Applications Tested:
Validated as the capture antibody in sandwich ELISA for mouse GM-CSF detection, using biotin-labeled MP1-31G6 (13-7332) for detection and recombinant mouse GM-CSF (14-8331) as the standard. Recommended capture concentration: 1–4 µg/mL. Include a standard curve (1000 pg/mL–8 pg/mL) in each ELISA plate.
Purity: >90% (SDS-PAGE)
Aggregation: <10% (HPLC)
Filtration: 0.2 µm post-manufacturing filtered
Target Information
The GM-CSF gene encodes a cytokine regulating macrophage and granulocyte production, differentiation, and function. The active protein exists as a homodimer in the extracellular space. Located at chromosome region 5q31, this gene cluster (including IL-4, IL-5, IL-13) is associated with 5q- syndrome and acute myelogenous leukemia. GM-CSF contributes to tissue inflammation and elevated levels are observed in SARS-CoV-2 patients with acute respiratory distress syndrome. Mice lacking GM-CSF or its receptor develop pulmonary alveolar proteinosis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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