Thermo Fisher Scientific CD201 (EPCR) Monoclonal Antibody (eBio1560 (1560)), PerCP-eFluor 710, eBioscience

Thermo Fisher Scientific CD201 (EPCR) Monoclonal Antibody (eBio1560 (1560)), PerCP-eFluor 710

Applications and Tested Dilution

• Application: Flow Cytometry (Flow)
• Tested Dilution: 0.5 µg/test
• View 8 publications

Product Specifications

Available Formats

• APC
• APC-eFluor 780
• Biotin
• Functional Grade
• PE
• Request custom conjugation

Product Specific Information

The eBio1560 monoclonal antibody reacts with mouse Endothelial Protein C Receptor (EPCR, CD201), a 25 kDa type I transmembrane protein expressed on endothelial cells.
EPCR is homologous to MHC class I/CD1 family proteins and acts as a ligand for Protein C, playing a key role in Protein C activation and regulation of coagulation and inflammation.
Deletion of EPCR leads to embryonic lethality, partly due to placental thrombosis.

EPCR expression identifies bone marrow cells with hematopoietic reconstitution activity comparable to conventional hematopoietic stem cells.
The eBio1560 antibody detects these stem cells, whereas eBiomRCR-16 should be used for endothelial cell detection.

Applications Reported: Flow cytometric analysis
Applications Tested: Flow cytometric analysis of mouse bone marrow cells at ≤0.5 µg/test (100 µL final volume).
Cell number: 10⁵–10⁸ cells/test (empirically determined).
Careful titration recommended for optimal performance.

Fluorochrome Information:

• PerCP-eFluor® 710 can replace PE-Cy5, PE-Cy5.5, or PerCP-Cy5.5.
• Emission: 710 nm; Excitation: 488 nm (Blue Laser).
• Recommended filters: 685 LP dichroic mirror + 710/40 band pass (alternative: 695/40 BP).
• Stable after fixation with 2% formaldehyde overnight at 4°C.
• Filtration: 0.2 µm post-manufacturing filtered.

Target Information

The encoded protein is a receptor for activated Protein C, a serine protease involved in blood coagulation.
It is an N-glycosylated type I membrane protein enhancing Protein C activation.
Mutations are associated with venous thromboembolism, myocardial infarction, and late fetal loss during pregnancy.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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