Thermo Fisher Scientific CD205 Monoclonal Antibody (205yekta), PerCP-eFluor 710, eBioscience
마우스 CD205(DEC-205) 인식 단클론 항체로, PerCP-eFluor 710 형광체 결합 제품입니다. 유세포분석(Flow Cytometry)에 최적화되어 있으며, 항원 포획 및 제시 연구에 활용됩니다. 488 nm 레이저로 여기되며, 710 nm에서 방출됩니다.
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Applications
- Flow Cytometry (Flow)
Tested dilution: 0.03 µg/test
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Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Mouse |
| Published Species | Mouse |
| Host / Isotype | Rat / IgG2a, kappa |
| Recommended Isotype Control | Rat IgG2a kappa Isotype Control (eBR2a), PerCP-eFluor™ 710, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 205yekta |
| Conjugate | PerCP-eFluor™ 710 |
| Excitation / Emission Max | 482 / 708 nm |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_1834423 |
Additional Available Formats
- Alexa Fluor 488
- APC
- Biotin
- NovaFluor Blue 660-120S
- NovaFluor Red 685
- NovaFluor Red 700
- NovaFluor Red 755
- NovaFluor Yellow 610
- NovaFluor Yellow 660
- NovaFluor Yellow 700
- NovaFluor Yellow 755
- PE
- PE-Cyanine7
- PE-eFluor 610
- Super Bright 436
- Super Bright 600
- Super Bright 645
- Request custom conjugation
Product Specific Information
Description:
The 205yekta antibody reacts with mouse CD205 (DEC-205), a 205 kDa integral membrane glycoprotein involved in antigen uptake, trafficking, and presentation, enhancing antigen-specific T cell immunity. CD205 is highly expressed on CD8+ dendritic cells and variably on bone marrow Gr1+ cells, Langerhans cells, bone marrow-derived dendritic cells (BMDCs), and thymic epithelial cells.
CD205 can bind PE-Cy5.5 antibody and streptavidin conjugates; therefore, this format should not be used on CD205+ dendritic cells.
Applications Reported: Flow cytometric analysis.
Applications Tested: Tested on mouse bone marrow cells at ≤0.03 µg/test (100 µL final volume). Optimize titration for best performance.
Fluorochrome Information:
PerCP-eFluor® 710 can replace PE-Cy5, PE-Cy5.5, or PerCP-Cy5.5.
- Excitation: 488 nm (Blue laser)
- Emission: 710 nm
- Recommended filters: 685 LP dichroic mirror and 710/40 band pass filter (695/40 acceptable)
PerCP-eFluor® 710 conjugates are stable after staining when samples are fixed with freshly prepared 2% formaldehyde overnight at 4°C.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
CD205 (LY75, Lymphocyte Antigen 75):
Integral membrane glycoprotein involved in antigen uptake, trafficking, and presentation that enhances antigen-specific T cell immunity. Highly expressed by CD8+ dendritic cells and variably by bone marrow Gr1+ cells, Langerhans cells, BMDCs, and thymic epithelial cells. Acts as an endocytic receptor directing captured antigens for processing and presentation, and may reduce B lymphocyte proliferation.
Associated Diseases: Pneumonic plague, lipid pneumonia.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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