Thermo Fisher Scientific CD206 (MMR) Monoclonal Antibody (19.2), PerCP-eFluor 710, eBioscience

Applications

Tested Application: Flow Cytometry (Flow)
Tested Dilution: 5 µL (0.06 µg)/test
Publications: View 29 publications

Product Specifications

Available Formats:

• Alexa Fluor 488
• Alexa Fluor 700
• APC
• Brilliant UV 805
• Brilliant Violet 421
• Brilliant Violet 711
• eFluor 450
• PE
• PE-Cyanine7
• Request custom conjugation

Product Specific Information

This 19.2 monoclonal antibody reacts with human CD206 (macrophage mannose receptor, MMR). CD206 is expressed on macrophages and dendritic cells, as well as on non-immune cells such as hepatic and lymphatic epithelia and kidney mesangial cells. It binds glycoproteins terminating in D-mannose, L-fucose, or N-acetylglucosamine, and is involved in antigen processing, presentation, cell migration, and intracellular signaling.

CD206 plays a key role in phagocytosis of pathogens such as Candida albicans, Leishmania, and Mycobacterium tuberculosis.

Applications Reported: Flow cytometric analysis
Applications Tested: Pre-titrated and tested on 3-day GM-CSF-cultured human peripheral blood monocytes. Use 5 µL (0.06 µg) per 100 µL cell sample (10⁵–10⁸ cells/test).

Fluorochrome Information:
PerCP-eFluor® 710 can replace PE-Cy5, PE-Cy5.5, or PerCP-Cy5.5.

• Excitation: 488 nm (Blue Laser)
• Emission: 710 nm
• Recommended filters: 685 LP dichroic mirror and 710/40 band pass filter (695/40 acceptable alternative)

Fixation Stability: Stable when exposed to freshly prepared 2% formaldehyde overnight at 4°C (evaluate for alternative fixation protocols).
Filtration: 0.2 µm post-manufacturing filtered.

Target Information

CD206 (MSR, Mannose receptor, MRC1) is a 175 kDa transmembrane protein belonging to pattern recognition receptors. It is predominantly expressed in tissue macrophages, dendritic cells, endothelial cells, and sperm cells. CD206 participates in innate and adaptive immune responses and mediates uptake of macromolecules including modified lipoproteins, AGEs, and amyloid β-protein.

It is implicated in processes related to atherosclerosis and Alzheimer’s disease. CD206 gene encodes three isoforms (1, 2, 3) via alternative splicing; type 1 and 2 are functional, while type 3 has a dominant negative effect by inhibiting endocytosis.

Diseases associated with CD206 dysfunction include leprosy and Gaucher’s disease.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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