Thermo Fisher Scientific Ki-67 Monoclonal Antibody (20Raj1), eBioscience
Ki-67 단백질을 인식하는 마우스 단클론 항체로, 세포 증식 마커 연구에 적합. IHC, ICC/IF, Flow Cytometry 등 다양한 응용 가능. 인간 및 개 Ki-67 반응성. 고순도(>90%) 정제, 4°C 보관.
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Thermo Fisher Scientific Ki-67 Monoclonal Antibody (20Raj1), eBioscience
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | - | View 2 publications |
| Immunohistochemistry (IHC) | - | View 7 publications |
| Immunohistochemistry (Paraffin) (IHC (P)) | 0.5–1 µg/mL | View 1 publication |
| Immunocytochemistry (ICC/IF) | 1:500–1:1,000 | View 3 publications |
| Flow Cytometry (Flow) | - | View 15 publications |
Product Specifications
| Property | Description |
|---|---|
| Species Reactivity | Dog, Human |
| Published Species | Dog, Human, Mouse, Non-human primate |
| Host / Isotype | Mouse / IgG1, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 20Raj1 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2865121 |
Available Conjugated Formats
- APC
- APC-eFluor 780
- Biotin
- Brilliant UV 395 / 563 / 615 / 737 / 805
- Brilliant Violet 421 / 480 / 605 / 650 / 711 / 786
- eFluor 450 / 506 / 570 / 615 / 660
- FITC
- PE / PE-Cyanine5.5 / PE-Cyanine7 / PE-eFluor 610
- PerCP-eFluor 710
- Request custom conjugation
Product Specific Information
The monoclonal antibody 20Raj1 recognizes the human Ki-67 protein. Two isoforms (345 and 395 kDa) are expressed in dividing cells. Ki-67 is detected during active phases of the cell cycle (G1, S, G2, and mitosis) but absent in resting cells (G0).
During interphase, Ki-67 localizes to the nucleus and redistributes to chromosomes during mitosis, associating with heterochromatin-bound proteins such as CBX3.
In tumor studies, Ki-67 serves as a marker for determining the fraction of proliferating cells.
This antibody also recognizes canine Ki-67.
Applications Reported: Immunocytochemistry and immunohistochemistry on formalin-fixed paraffin-embedded tissues.
Applications Tested: ICC on paraformaldehyde-fixed MCF7, MDCK, and MDA-MB-175 cell lines; IHC on FFPE human skin using citrate buffer antigen retrieval.
Recommended concentration ≤0.5–1 µg/mL, with titration for optimal performance.
Purity: >90% (SDS-PAGE)
Aggregation: <10% (HPLC)
Filtration: 0.2 µm post-manufacturing filtered
Target Information
Ki-67 is a nuclear protein expressed during late G1, S, G2, and M phases of the cell cycle. It associates with euchromatin at the perichromosomal layer, centromeric heterochromatin, and nucleolus.
Ki-67 shows cell cycle-dependent localization: perinucleolar in G1, nuclear matrix in G2, and chromosomal in M phase.
It is widely used as a proliferation marker since it is absent in G0 cells but increases through mitosis.
Ki-67 antibodies are useful for determining the growing fraction in neoplasms and have prognostic value comparable to thymidine-labelling index.
Commonly used as a neuronal marker for cell cycling and proliferation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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