Thermo Fisher Scientific Aurora Kinase B Polyclonal Antibody
Aurora Kinase B 단백질을 인식하는 Thermo Fisher Scientific의 토끼 폴리클로날 항체입니다. Western blot, ICC/IF, ELISA, IP 등에 사용 가능하며 인간 단백질에 반응합니다. 고순도 친화 크로마토그래피 정제, 액상 형태로 -20°C 보관합니다.
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Thermo Fisher Scientific Aurora Kinase B Polyclonal Antibody
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500 – 1:2,000 |
| Immunocytochemistry (ICC/IF) | Assay-dependent |
| ELISA | 1:5,000 – 1:20,000 |
| Immunoprecipitation (IP) | 1:500 – 1:2,000 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide corresponding to an internal region near aa 225–250 of Human Aurora Kinase B protein |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1.39 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | 0.02M potassium phosphate, pH 7.2, with 0.15M NaCl |
| Contains | 0.01% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- Store vial at -20°C prior to opening.
- Aliquot contents and freeze at -20°C or below for extended storage.
- Avoid cycles of freezing and thawing.
- Centrifuge product if not completely clear after standing at room temperature.
- Stable for several weeks at 4°C as an undiluted liquid.
- Dilute only prior to immediate use.
Reactivity occurs against human Aurora Kinase B protein. No reaction is expected against Aurora Kinase A.
BLAST analysis indicates 100% sequence homology for this protein from human, mouse, rat, cow, pig, dog, and chimpanzee.
Cross-reactivity with Aurora Kinase B from other sources is not known.
Target Information
Aurora kinases associate with microtubules during chromosome movement and segregation.
AURKB (Aurora B) localizes to microtubules near kinetochores, specifically to K-fibers.
This complex functions in chromosome alignment and separation, histone modification, and cytokinesis.
The Chromosomal Passenger Complex (CPC) includes:
- Aurora B kinase
- INCENP
- Survivin
- Borealin
Two CPCs exist during mitosis:
- Full complex containing all four members
- INCENP–AurB complex responsible for histone H3 modification
AurB is overexpressed in various tumors, including breast and colon cancers, and may be linked to Aurora-A kinase expression through feedback regulation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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