Thermo Fisher Scientific PC2 Polyclonal Antibody
Human PC2 단백질을 인식하는 Rabbit Polyclonal 항체로, WB, ICC/IF, ELISA, IP 등 다양한 응용에 사용 가능. Affinity chromatography로 정제되었으며, -20°C에서 보관. 연구용으로 적합.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunocytochemistry (ICC/IF) | 1:100–1:400 |
| ELISA | 1:10,000–1:40,000 |
| Immunoprecipitation (IP) | 1:1,000–1:4,000 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region near aa 90–115 of Human PC2 protein. |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1.36 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | 0.02M potassium phosphate, pH 7.2, with 0.15M NaCl |
| Contains | 0.01% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use.
Reactivity occurs against human PC2 protein.
Target Information
Polycomb group (PcG) proteins are chromatin-associated proteins important for embryonic and adult stem cell self-renewal and maintenance. At least two distinct human PcG complexes have been identified.
Polycomb repressive complex I (PRC1) contains CBX4/PC2, HPH1, and RING domain-containing proteins (RING1, RING2, and BMI1).
CBX4 (Chromobox protein homolog 4 or PC2) functions as a transcriptional suppressor and acts as an E3 SUMO-protein ligase facilitating SUMO1 conjugation. Depletion of CBX4 results in decreased cellular resistance to ionizing radiation, indicating its involvement in DNA damage response.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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