Thermo Fisher Scientific Profilin 1 Recombinant Rabbit Monoclonal Antibody (JA30-13)
Profilin 1 단백질을 인식하는 재조합 토끼 단클론 항체로, Western blot, IHC, ICC, Flow cytometry 등에 사용 가능. 높은 특이성과 재현성을 제공하며, HEK293 발현 시스템에서 생산된 IgG 형 항체. 연구용으로만 사용.
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:500–1:2,000 | View 1 publication |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 | - |
| Immunocytochemistry (ICC/IF) | 1:50–1:200 | View 1 publication |
| Flow Cytometry (Flow) | 1:50–1:100 | - |
| Immunoprecipitation (IP) | - | View 1 publication |
Product Specifications
| Item | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Published Species | Human, Mouse |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | JA30-13 |
| Immunogen | Synthetic peptide within Human Profilin 1 (aa 91–140) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | TBS, pH 7.4, with 40% Glycerol, 0.05% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2809960 |
Product Specific Information
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The specific antibody DNA sequences from immunoreactive rabbits are cloned and screened to select optimal production candidates.
Advantages include:
- High specificity and sensitivity
- Lot-to-lot consistency
- Animal origin-free formulation
- Broader immunoreactivity due to the rabbit immune repertoire
Target Information
Profilin is a small (12–15 kDa) phosphoinositide and poly-L-proline binding protein involved in signal transduction and actin filament dynamics.
Mammalian profilins include Profilin I and Profilin II, which have distinct expression patterns:
- Profilin I: Highly expressed in most tissues except skeletal muscle
- Profilin II: Predominantly expressed in brain, lower levels in skeletal muscle, uterus, and kidney
Profilin is mainly cytosolic, enriched in dynamic membrane regions such as the leading edge and ruffling membranes.
For Research Use Only. Not for use in diagnostic procedures or resale without authorization.
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