Thermo Fisher Scientific Phospho-IRS1 (Ser323) Polyclonal Antibody
Phospho-IRS1 (Ser323) 항체로 인간, 마우스, 랫트 시료에서 인산화된 IRS-1 단백질 검출에 적합. Western blot, IHC, ICC/IF에 사용 가능. 항원 친화 크로마토그래피로 정제된 고순도 rabbit polyclonal 항체. 연구용으로만 사용.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:1,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 |
| Immunocytochemistry (ICC/IF) | 1:50–1:200 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic phosphopeptide derived from human IRS-1 around the phosphorylation site of Serine 323 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2553694 |
Product Specific Information
This antibody detects endogenous IRS-1 protein at molecular weights of approximately 132 and 180 kDa.
Purity is >95% by SDS-PAGE.
Target Information
IRS-1 (Insulin Receptor Substrate 1) is a major substrate of the insulin receptor and is phosphorylated in response to insulin, IGF-1, and IL-4 stimulation. It undergoes phosphorylation on serine, threonine, and tyrosine residues across various tissues. Casein kinase II mediates part of the insulin-stimulated serine/threonine phosphorylation of IRS-1. Identified phosphorylation sites include Thr 502 (major site) and Ser 99 (additional site) in rat IRS-1.
IRS-1 contains three putative binding sites for 14-3-3 proteins (Ser 270, Ser 374, Ser 641), with the Ser 270 motif located in the phosphotyrosine-binding domain responsible for interaction with the insulin receptor.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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