Thermo Fisher Scientific Phospho-MDM2 (Ser166) Polyclonal Antibody

Applications

Western Blot (WB)

• Tested Dilution: 1:500–1:1,000

Immunohistochemistry (Paraffin) (IHC (P))

• Tested Dilution: 1:50–1:200

Product Specifications

Product Specific Information

This antibody detects endogenous protein at molecular weights of approximately 55 and 90 kDa.
Purity is >95% by SDS-PAGE.

Target Information

MDM2 is a ubiquitin ligase for p53 and plays a central role in regulating p53 stability. Located on chromosome 12q, phosphorylation of MDM2 at Ser166 and Ser186 by Akt enhances its interaction with p300, promoting MDM2-mediated ubiquitination and degradation of p53.
Phosphorylation also inhibits binding to p19ARF, further increasing p53 degradation. Overexpression of MDM2 can inactivate p53 and reduce its tumor suppressor function.
MDM2 also participates in apoptosis and cell cycle regulation and is overexpressed in various human malignancies, including soft tissue carcinomas and breast cancer.
Beyond p53, MDM2 interacts with proteins such as retinoblastoma 1 and ribosomal protein L5, influencing cell cycle and tumorigenesis.
More than 40 alternatively spliced transcript variants have been identified from tumor and normal tissues.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.