Thermo Fisher Scientific Axl Monoclonal Antibody (C.995.0)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

-

Immunocytochemistry (ICC/IF)

5 µg

-

Immunoprecipitation (IP)

1:50

-

Product Specifications

Species Reactivity

Human, Non-human primate

Host/Isotype

Rabbit / IgG

Class

Monoclonal

Type

Antibody

Clone

C.995.0

Immunogen

Recombinant human fragment of the Axl protein

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

64 µg/mL

Purification

Affinity chromatography

Storage buffer

0.01M HEPES, pH 7.5, with 0.15M NaCl, 100µg/mL BSA, 50% glycerol

Contains

<0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice

RRID

AB_10986568

Product Specific Information

It is not recommended to aliquot this antibody.

This antibody is not cross-reactive with related proteins.

Target Information

AXL is a chronic myelogenous leukemia-associated oncogene that is also associated with colon cancer and melanoma. The AXL protein is a receptor tyrosine kinase that transduces signals from the extracellular matrix into the cytoplasm by binding growth factors like vitamin K-dependent protein growth-arrest-specific gene 6, and is involved in the stimulation of cell proliferation. TAM receptors are also involved in phagocytosis of apoptotic cells. Mice lacking all three TAM receptors have several degenerative phenotypes linked to inefficient removal of apoptotic cells and membranes (e.g., in the retina and the male reproductive tract) and develop a severe autoimmune phenotype akin to systemic lupus erythematosus, including the production of broad spectrum auto-antibodies. In addition, Axl may function as a putative entry receptor for filoviruses. Axl is also used to identify a specific subpopulation of human blood dendritic cells, also referred to as Siglec 6+/Axl+ dendritic cells. Cellular expression of Axl can be upregulated by TLR ligands, such as LPS or poly I:C. Soluble Axl is generated by proteolytic cleavage of the membrane form. Increased plasma levels may be indicative of inflammation and cancer.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.