Thermo Fisher Scientific RNF2 Recombinant Rabbit Monoclonal Antibody (13H1L14)
RNF2 단백질을 표적하는 Thermo Fisher Scientific의 재조합 토끼 단클론 항체입니다. Western blot 및 ICC/IF에 적합하며, 높은 특이성과 민감도를 제공합니다. 인비트로 발현 시스템으로 생산되어 로트 간 일관성과 동물 유래 성분이 없습니다.
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Applications
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500 |
| Immunocytochemistry (ICC/IF) | 1:100 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host/Isotype | Rabbit / IgG |
| Expression System | Expi293 |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | 13H1L14 |
| Immunogen | Peptides corresponding to human RNF2 (aa183–aa201, aa155–aa174) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Protein A |
| Storage Buffer | PBS, pH 7.4 |
| Contains | 0.09% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2762381 |
Product Specific Information
This antibody is predicted to react with Monkey, Mouse, Rat, and Pig.
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems developed by cloning specific antibody DNA sequences from immunoreactive rabbits. Individual clones are screened to select the best candidates for production.
Advantages of recombinant rabbit monoclonal antibodies:
- Better specificity and sensitivity
- Lot-to-lot consistency
- Animal origin-free formulations
- Broader immunoreactivity due to larger rabbit immune repertoire
Target Information
RNF2 (E3 ubiquitin-protein ligase RING2) mediates monoubiquitination of Lys-119 of histone H2A (H2AK119Ub), playing a central role in histone code and gene regulation. Its ligase activity is enhanced by BMI1/PCGF4. H2AK119Ub acts as a tag for epigenetic transcriptional repression and participates in X chromosome inactivation in female mammals.
RNF2 is an essential component of the Polycomb group (PcG) multiprotein PRC1-like complex, which maintains transcriptional repression of genes such as Hox throughout development via chromatin remodeling and histone modification.
For Research Use Only. Not for use in diagnostic procedures or resale without authorization.
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