Thermo Fisher Scientific Ep-CAM/CD326 Recombinant Mouse Monoclonal Antibody (rEGP40, 1372)
Ep-CAM/CD326 표적의 재조합 마우스 단클론 항체로, HEK293 세포에서 발현된 IgG1 형 항체입니다. WB, IHC(P), Peptide Array에 사용 가능하며, 단백질 A/G로 정제된 액상 형태입니다. 인간 EpCAM 단백질을 면역원으로 하며 연구용으로 적합합니다.
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Applications
| Application | Tested Dilution | Notes |
|---|---|---|
| Western Blot (WB) | 1–2 µg/mL | |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1–2 µg/mL | |
| Peptide Array | Assay-dependent |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Dog, Cat, Human |
| Host / Isotype | Mouse / IgG1, kappa |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | rEGP40, 1372 |
| Immunogen | Recombinant human EpCAM protein fragment from extracellular domain (around aa 100–224) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A/G |
| Storage Buffer | PBS, pH 7.4 |
| Contains | No preservative |
| Storage Conditions | −20°C or −80°C if preferred |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- This antibody does not react with Dog or Cat.
- For Immunohistochemistry (PFA-fixed): incubate antibody for 30 min at room temperature.
- Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA (pH 9.0) for 45 min at 95°C, followed by cooling at room temperature for 20 min.
Target Information
Ep-CAM (epithelial adhesion molecule, epithelial specific antigen, ESA) is a transmembrane glycoprotein expressed in epithelial cells (~40 kDa). It functions as a homotypic calcium-independent cell adhesion molecule and influences cell cycle, proliferation, and metabolism by inducing proto-oncogene c-myc and cyclins A and E.
Ep-CAM-mediated adhesions negatively regulate cadherin-mediated adhesions, affecting epithelial differentiation and growth. Overexpression is associated with enhanced epithelial proliferation and is highly expressed in human carcinomas, serving as a marker for epithelial tumors.
Ep-CAM is localized on the baso-lateral cell surface of most simple epithelia and many carcinoma types, and can distinguish adenocarcinomas from pleural mesotheliomas.
For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.
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