Thermo Fisher Scientific Ep-CAM/CD326 Recombinant Mouse Monoclonal Antibody (rEGP40, 1372)
Ep-CAM/CD326 표적 재조합 마우스 단클론 항체로, Western blot 및 IHC(P) 등 다양한 응용 가능. HEK293 세포에서 발현된 IgG1, kappa 항체이며, Protein A/G로 정제됨. 인간 EpCAM 단백질(aa 100-224) 기반 면역원 사용. 연구용으로만 사용.
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Applications
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1–2 µg/mL |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1–2 µg/mL |
| Peptide Array (Array) | Assay-dependent |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Dog, Cat, Human |
| Host / Isotype | Mouse / IgG1, kappa |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | rEGP40, 1372 |
| Immunogen | Recombinant human EpCAM protein fragment from extracellular domain (around aa 100–224) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 200 µg/mL |
| Purification | Protein A/G |
| Storage Buffer | PBS, pH 7.4, with 0.05% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- This antibody does not react with Dog or Cat.
- For immunohistochemistry (PFA fixed): incubate antibody for 30 min at room temperature.
- Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C, followed by cooling at room temperature for 20 min.
Target Information
Ep-CAM (epithelial adhesion molecule, epithelial specific antigen, ESA) is a transmembrane glycoprotein (~40 kDa) expressed in epithelial tissues. It functions as a calcium-independent cell adhesion molecule and influences cell cycle, proliferation, and metabolism through induction of proto-oncogene c-myc and cyclins A/E.
Ep-CAM-mediated adhesions negatively regulate cadherin-mediated adhesions, impacting epithelial differentiation and growth. Overexpression is associated with enhanced epithelial proliferation and is a marker for tumors of epithelial lineage.
Ep-CAM is expressed on baso-lateral surfaces in most simple epithelia and carcinoma types, and distinguishes adenocarcinomas from pleural mesotheliomas.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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