Thermo Fisher Scientific Ep-CAM/CD326 Recombinant Mouse Monoclonal Antibody (rEGP40, 1110)
Ep-CAM/CD326 단백질을 인식하는 마우스 유래 재조합 단일클론 항체. IHC(P) 및 Peptide Array에 사용 가능. 인간, 마우스, 랫트 반응성. HEK293 세포에서 발현된 IgG1, kappa 항체로 Protein A/G 정제. 보존제 무첨가 PBS 용액 형태로 제공.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Immunohistochemistry (Paraffin) (IHC (P)) | 1–2 µg/mL |
| Peptide Array (Array) | Assay-dependent |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Mouse / IgG1, kappa |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | rEGP40, 1110 |
| Immunogen | Recombinant human EpCAM fragment from the cytoplasmic domain (around aa 289–314) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A/G |
| Storage Buffer | PBS, pH 7.4 |
| Contains | No preservative |
| Storage Conditions | -20°C or -80°C if preferred |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- For Immunohistochemistry (PFA-fixed):
Incubate antibody for 30 minutes at room temperature.
Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA (pH 9.0) for 45 minutes at 95°C, followed by cooling at room temperature for 20 minutes.
Target Information
Ep-CAM (epithelial adhesion molecule, ESA) is a transmembrane glycoprotein (~40 kDa) expressed in epithelial tissues. It functions as a calcium-independent cell adhesion molecule and influences cell cycle, proliferation, and metabolism by inducing proto-oncogene c-myc and cell cycle genes cyclin A and E.
Ep-CAM-mediated adhesions negatively regulate cadherin-mediated adhesions, affecting epithelial differentiation and growth. Overexpression of Ep-CAM is associated with enhanced epithelial proliferation and is highly expressed in human carcinomas, serving as a marker for epithelial-derived tumors. It is localized on the basolateral surface of most simple epithelia and many carcinoma types and can distinguish adenocarcinomas from pleural mesotheliomas.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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