Thermo Fisher Scientific CYR61 Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:200

-

Immunocytochemistry (ICC/IF)

1:50-1:100

-

Neutralization (Neu)

-

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Product Specifications

Published species

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

A synthetic peptide made to the human CYR61 protein sequence (between residues 250-350).

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Storage conditions

4° C, do not freeze

Shipping conditions

Wet ice

RRID

AB_2088728

Product Specific Information

This antibody does not react with mouse and rat samples despite 94% sequence homology.

Suggested positive control: antigen standard for CYR61 (transient overexpression lysate), MDA-MB-231 breast cancer cell lysate.

Target Information

CYR61 is a secreted heparin binding protein, encoded by a growth factor inducible immediate-early gene that associates with the extracellular matrix and connective tissue. CYR61 is a member of a distinct family of angiogenic and vasculogenic regulators designated CCN proteins, which includes connective tissue growth factor (CTGF) and the mouse CYR61 homolog, Fisp12. As an angiogenic inducer, CYR61 binds to the cell surface receptor integrin alphavbeta3, where it then stimulates cell adhesion and migration and promotes DNA synthesis of human vascular endothelial cells. Expression of CYR61 is elevated during vessel growth, wound healing and chondrocyte differentiation. CYR61 is also detected in a wide variety of tumors as it induces tumor growth and functions as a marker of tumor progression.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.