Thermo Fisher Scientific Cytokeratin 14 (Squamous Cell Marker) Monoclonal Antibody (SPM263)
Cytokeratin 14 단백질을 표적하는 단클론 항체로, 편평상피세포 마커로 활용 가능. 인간, 생쥐, 랫트 시료에서 반응하며 IHC 및 ICC/IF 실험에 적합. 단백질 A/G로 정제된 액상 형태, PBS buffer에 보존. 연구용으로만 사용.
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Thermo Fisher Scientific Cytokeratin 14 (Squamous Cell Marker) Monoclonal Antibody (SPM263)
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Immunohistochemistry (Paraffin) (IHC (P)) | Assay-dependent |
| Immunohistochemistry (PFA fixed) (IHC (PFA)) | 1–2 µg/mL |
| Immunocytochemistry (ICC/IF) | 2–4 µg/mL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Mouse / IgG3, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | SPM263 |
| Immunogen | A synthetic peptide of 15 amino acids from the C-terminus of human keratin 14 (range 457–472, UniProt ID: P02533-1) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A/G |
| Storage Buffer | PBS, pH 7.4 |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Wet ice |
Target Information
Cytokeratins (CK) are intermediate filaments of epithelial cells, found in both keratinizing tissue (e.g., skin) and non-keratinizing cells (e.g., mesothelial cells). At least 20 different CKs have been identified. CKs are divided into two classes: type I (acidic polypeptides) and type II (basic polypeptides).
Keratin 14, a type I low molecular weight keratin, forms a complex with keratin 5 and is expressed in stratified epithelial cells. It is useful for identifying squamous cell carcinomas and is considered a prognostic marker in breast carcinomas. Both acidic and basic keratins are expressed in all epithelial cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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