Thermo Fisher Scientific GAPDH Polyclonal Antibody
GAPDH 단백질을 인식하는 Sheep Polyclonal 항체로, Western Blot에 적합합니다. Human, Mouse, Rat에 반응하며, 단백질 로딩 컨트롤용으로 널리 사용됩니다. 동결건조 형태로 제공되며 4°C 또는 -20°C에서 보관 가능합니다.
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Applications
Western Blot (WB)
- Tested Dilution: 1:70,000–1:100,000
- Publications: 2 (Miscellaneous PubMed)
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Published Species | Not Applicable |
| Host / Isotype | Sheep / Ig |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A synthetic peptide from the C-terminal of rat GAPDH conjugated to an immunogenic carrier protein |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | Not determined |
| Storage Buffer | Whole serum |
| Contains | No preservative |
| Storage Conditions | Store at 4°C short term. For long-term storage, store at -20°C, avoiding freeze/thaw cycles. Glycerol (1:1) may be added for stability. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- Reconstitute in 100 µL of sterile water.
- Centrifuge to remove any insoluble material.
- The antigen is homologous in mouse and shares 90% identity with human sequence.
- Specificity: GAPDH.
Target Information
GAPDH (Glyceraldehyde-3-phosphate dehydrogenase) is a catalytic enzyme involved in glycolysis. It exists as a tetramer of identical 37-kDa subunits and catalyzes the reversible reduction of 1,3-bisphosphoglycerate to glyceraldehyde 3-phosphate in the presence of NADPH.
Beyond glycolysis, GAPDH participates in:
- DNA replication and repair
- Nuclear RNA export
- Membrane fusion and microtubule bundling
- Gene expression regulation during apoptosis
- Cellular processes in neurodegenerative diseases and cancer
GAPDH interacts with various proteins, including amyloid precursor protein (linked to Alzheimer’s disease) and Huntingtin (linked to Huntington’s disease). Due to its high expression in most tissues, GAPDH is widely used as a protein loading control in Western blot analysis.
For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.
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