Thermo Fisher Scientific GAPDH Polyclonal Antibody
GAPDH 단백질을 인식하는 sheep polyclonal antibody로, WB 및 IHC에 적합합니다. Human, Mouse, Rat 반응성 보유하며 PBS buffer에 lyophilized 형태로 제공됩니다. 연구용으로 사용되며 Western blot의 loading control로 활용됩니다.
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Applications
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 10–50 µg/mL | - |
| Immunohistochemistry (IHC) | 10–50 µg/mL | - |
| Miscellaneous PubMed (Misc) | - | View 2 publications |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Published Species | Not Applicable |
| Host / Isotype | Sheep / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A synthetic peptide from the C-terminal of rat GAPDH conjugated to a carrier protein |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | Not Determined |
| Purification | Ammonium sulfate precipitation |
| Storage Buffer | PBS |
| Contains | No preservative |
| Storage Conditions | Store at 4°C (short term). For long term, store at -20°C and avoid freeze/thaw cycles. Glycerol (1:1) may be added for stability. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- Reconstitute in 500 µL of sterile water.
- Centrifuge to remove any insoluble material.
- The antigen is homologous in mouse and shares 90% identity with the human sequence.
- Specificity: GAPDH.
Target Information
GAPDH (Glyceraldehyde-3-phosphate dehydrogenase) is a catalytic enzyme involved in glycolysis. It exists as a tetramer of identical 37-kDa subunits and catalyzes the reversible reduction of 1,3-bisphosphoglycerate to glyceraldehyde 3-phosphophate in the presence of NADPH.
Beyond its glycolytic role, GAPDH participates in various cellular processes such as DNA replication, DNA repair, nuclear RNA export, membrane fusion, and microtubule bundling. It is implicated in apoptosis, neurodegenerative diseases (e.g., Alzheimer’s and Huntington’s), and cancer-related pathways.
Due to its high expression in most tissues, GAPDH is commonly used as a protein loading control in Western blot analysis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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