Thermo Fisher Scientific CD64 Monoclonal Antibody (10.1), NovaFluor Blue 660-120S, eBioscience

Thermo Fisher Scientific CD64 Monoclonal Antibody (10.1), NovaFluor Blue 660-120S, eBioscience

Applications

• Flow Cytometry (Flow)

Tested Dilution: 5 µL (0.6 µg)/test

Product Specifications

Additional Formats

• Unconjugated
• APC
• APC-eFluor 780
• eFluor 450
• FITC
• Functional Grade
• PE
• PE-Cyanine7
• PE-eFluor 610
• PerCP-eFluor 710
• Super Bright 436
• Super Bright 600
• Request custom conjugation

Product Specific Information

The 10.1 monoclonal antibody reacts with human CD64 (FcRI), a 75 kDa type I transmembrane protein. CD64 is the high-affinity receptor for IgG and is involved in antibody-dependent cell-mediated cytotoxicity (ADCC), phagocytosis, and regulation of cytokine production. Monocytes and macrophages express CD64, while mature granulocytes and lymphocytes are negative.

Applications Reported:

• Flow cytometric analysis
• Immunohistology staining of frozen tissue sections

Applications Tested:
This antibody has been tested by flow cytometric analysis of normal human peripheral blood cells. Recommended usage ≤ 5 µL (0.6 µg)/test. A test is defined as the amount of antibody staining a cell sample in a final volume of 100 µL. Cell number may range from 10⁵ to 10⁸ cells/test. Titrate antibody for optimal performance.

Each product contains:

• 1 vial of NovaFluor conjugate
• 1 vial of CellBlox Plus Blocking Buffer

Important Notes:

• NovaFluor dyes are not compatible with DNA intercalating viability dyes (e.g., propidium iodide, 7-AAD, DAPI).
• Use Invitrogen LIVE/DEAD Fixable Dead Cell stains for viability assays.
• CellBlox Plus Blocking Buffer (Cat. No. C001T06F01) is required for optimal staining.
• Add 5 µL per sample before combining with cells for best results.
• For single-color controls, use 5 µL per 100 µL cell sample containing 10³–10⁸ cells.

NovaFluor conjugates utilize Phiton technology based on novel nucleic acid dye structures engineered for controlled fluorescent signatures minimizing spillover and spread.

Excitation: 509 nm
Emission: 665 nm
Laser: 488 nm (Blue)

Target Information

CD64 (FCGR1A) encodes a high-affinity Fc-gamma receptor involved in immune response. It is one of three related gene family members on chromosome 1. CD64 dysfunction has been associated with cervical adenitis and tetrasomy 21.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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