Thermo Fisher Scientific CD47 Monoclonal Antibody (2D3), NovaFluor Yellow 660, eBioscience

Thermo Fisher Scientific CD47 Monoclonal Antibody (2D3), NovaFluor Yellow 660, eBioscience

Applications

• Flow Cytometry (Flow)

Tested Dilution: 5 µL (0.6 µg)/test

Product Specifications

Available Formats

• Unconjugated
• FITC
• NovaFluor Blue 510
• NovaFluor Blue 585
• NovaFluor Blue 610-70S
• NovaFluor Blue 660-120S
• NovaFluor Blue 690
• NovaFluor Red 710
• NovaFluor Red 725
• NovaFluor Yellow 570
• NovaFluor Yellow 730
• NovaFluor Yellow 755
• Super Bright 436
• Request custom conjugation available

Product Specific Information

The monoclonal antibody 2D3 reacts to CD47, also known as integrin-associated protein (IAP) and neurophilin. CD47 is a glycosylated five-transmembrane protein involved in adhesion through interactions with SIRP (signal regulator protein) and integrins such as CD51/CD61 and CD41/CD61. It mediates bi-directional signaling affecting neural synaptic activity and macrophage phagocytosis. CD47 is also the receptor for thrombospondin.

CD47 expression is found in most hematopoietic cells (T/B cells, monocytes, platelets, erythrocytes) and also in non-hematopoietic cells.

Each product includes:

• 1 vial of NovaFluor conjugate
• 1 vial of CellBlox Plus Blocking Buffer

Applications Reported: Flow cytometric analysis
Applications Tested: Pre-titrated for flow cytometry on normal human peripheral blood cells at 5 µL (0.6 µg) per test (100 µL final volume). Cell number range: 10⁵–10⁸ cells/test.

Important Notes:

• NovaFluor dyes are not compatible with DNA intercalating viability dyes (e.g., propidium iodide, 7-AAD, DAPI).
• Use Invitrogen LIVE/DEAD Fixable Dead Cell stains for viability assays.
• CellBlox Plus Blocking Buffer (Cat. No. C001T06F01) is required for optimal staining. Add 5 µL per sample before combining with cells.
• For single-color controls, use 5 µL per 100 µL sample containing 10³–10⁸ cells.

NovaFluor conjugates utilize Phiton™ technology with engineered fluorescent signatures designed to minimize spillover and spread.
Excitation: 552 nm; Emission: 663 nm; Laser: 561 nm (Yellow).

Target Information

CD47 (integrin-associated protein, IAP) is a ubiquitously expressed transmembrane glycoprotein that interacts with integrins and modulates signaling pathways, including G protein activation and calcium flux upon adhesion. It acts as a receptor for thrombospondin and plays roles in membrane transport and signal transduction. CD47 expression is broad but reduced on Rh erythrocytes. Interaction with CD172a (SIRPα) prevents phagocytosis of CD47-positive cells and influences migration, adhesion, activation, mechanical signaling, and neuronal development, particularly in synapse-rich brain and retina regions.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without authorization.

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