Thermo Fisher Scientific ATRX/RAD54 (Alpha Thalassemia Mental Retardation) Recombinant Mouse Monoclonal Antibody (rATRX, 3446)

Applications

Immunohistochemistry (Paraffin) (IHC (P))

• Tested Dilution: 1–2 µg/mL

Product Specifications

Product Specific Information

• For Immunohistochemistry (PFA fixed): Incubate antibody for 30 minutes at room temperature.
• Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA (pH 9.0) for 45 minutes at 95°C, followed by cooling at room temperature for 20 minutes.

Target Information

ATRX encodes a protein that contains an ATPase/helicase domain and belongs to the SWI/SNF family of chromatin remodeling proteins.
Mutations in this gene are associated with X-linked mental retardation (XLMR) syndrome, often accompanied by alpha-thalassemia (ATRX) syndrome.
These mutations can cause changes in DNA methylation patterns, linking chromatin remodeling, DNA methylation, and gene expression during development.
ATRX protein undergoes cell cycle-dependent phosphorylation, regulating nuclear matrix and chromatin association, and is involved in gene regulation during interphase and chromosomal segregation in mitosis.
Multiple alternatively spliced transcript variants encoding distinct isoforms have been reported.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.