Thermo Fisher Scientific H3K4ac Recombinant Rabbit Monoclonal Antibody (RM149), ChIP-Verified
H3K4ac에 특이적인 재조합 토끼 단클론 항체로, ChIP 검증 완료. Western blot, ICC/IF, ELISA, ChIP 등 다양한 응용에 적합. 높은 특이성과 일관된 로트 간 재현성을 제공하며, 동물 유래 성분이 없는 포뮬레이션.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 0.25–1 µg/mL |
| Immunocytochemistry (ICC/IF) | 0.5–2 µg/mL |
| ELISA | 0.2–1 µg/mL |
| ChIP assay (ChIP) | 1–5 µg |
| Luminex (LUM) | 0.05–0.5 µg/mL |
| Peptide Array (Array) | 0.25 µg/mL |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse |
| Published Species | Human |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | RM149 |
| Immunogen | Acetyl-peptide corresponding to the Acetyl-Histone H3 (Lys4) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | PBS, pH 7.2–7.4, with 50% glycerol, 1% BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | −20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2661896 |
Product Specific Information
This antibody reacts specifically to Histone H3 acetylated at Lysine 4 (K4ac) and shows no cross-reactivity with other acetylated lysines in histone H3.
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems by cloning specific antibody DNA sequences from immunoreactive rabbits. Clones are screened to select optimal candidates for production.
Advantages of recombinant rabbit monoclonal antibodies:
- Superior specificity and sensitivity
- Lot-to-lot consistency
- Animal origin-free formulation
- Broader immunoreactivity due to rabbit immune repertoire
Click here for Master Lot linking information
Target Information
Histone H3 is one of the core histone proteins forming nucleosomes in eukaryotic chromatin. Together with other histones, it binds DNA to form nucleosome structures essential for chromatin organization.
Histones play key roles in:
- Transcription regulation
- DNA repair and replication
- Chromosomal stability
The N-terminal tail of histone H3 undergoes multiple post-translational modifications such as methylation, acetylation, and phosphorylation, influencing chromatin structure and gene expression.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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