Thermo Fisher Scientific H3K56me1 Recombinant Rabbit Monoclonal Antibody (RM180)
Histone H3의 Lys56 단일 메틸화(K56me1)를 인식하는 재조합 토끼 단클론 항체. Western blot, ELISA, Luminex 분석에 적합. 높은 특이성과 로트 간 일관성 제공. 동물 유래 성분 없는 포뮬레이션으로 안정적 연구용 시약.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1–2 µg/mL |
| ELISA | 0.2–1 µg/mL |
| Luminex | 0.1–0.5 µg/mL |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | RM180 |
| Immunogen | Monomethyl-peptide corresponding to Monomethyl-Histone H3 (Lys56) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | PBS, pH 7.2–7.4, with 50% glycerol, 1% BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | –20°C, avoid freeze/thaw cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2661901 |
Product Specific Information
This antibody reacts specifically to Histone H3 monomethylated at Lysine 56 (K56me1) and shows no cross-reactivity with non-modified Lysine 56 or other methylations in histone H3.
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems by cloning specific antibody DNA sequences from immunoreactive rabbits. Individual clones are screened to select optimal candidates for production.
Advantages of recombinant rabbit monoclonal antibodies:
- Superior specificity and sensitivity
- Lot-to-lot consistency
- Animal origin-free formulation
- Broader immunoreactivity due to the rabbit immune repertoire
Target Information
Histone H3 is a DNA-binding protein found in the chromatin of all eukaryotic cells. Together with four core histone proteins, it forms the nucleosome structure. Histones play essential roles in transcription regulation, DNA repair, replication, and chromosomal stability. Post-translational modifications such as methylation, acetylation, and phosphorylation of the N-terminal tail of histone H3 influence chromatin structure and gene expression.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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