Thermo Fisher Scientific H3K56ac Recombinant Rabbit Monoclonal Antibody (RM179)
H3K56ac에 특이적인 재조합 토끼 단클론 항체로, Western blot, IHC, ICC/IF, ELISA, Luminex 등 다양한 응용에 적합합니다. 높은 특이성과 재현성을 제공하며, 로트 간 일관성이 우수합니다. 연구용으로 인간 시료에 반응합니다.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1–2 µg/mL |
| Immunohistochemistry (IHC) | 1–10 µg/mL |
| Immunocytochemistry (ICC/IF) | 0.5–2 µg/mL |
| ELISA | 0.5–1 µg/mL |
| Luminex (LUM) | 0.5–2 µg/mL |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | RM179 |
| Immunogen | Acetyl-peptide corresponding to Acetyl-Histone H3 (Lys56) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | PBS (pH 7.2–7.4) with 50% glycerol, 1% BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | −20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2661916 |
Product Specific Information
This antibody reacts specifically with Histone H3 acetylated at Lysine 56 (H3K56ac) and shows no cross-reactivity with other acetylated lysines in histone H3.
Recombinant rabbit monoclonal antibodies are generated through in vitro expression systems by cloning specific antibody DNA sequences from immunoreactive rabbits. Individual clones are screened to select the best candidates for production.
Advantages of recombinant rabbit monoclonal antibodies:
- High specificity and sensitivity
- Lot-to-lot consistency
- Animal origin-free formulation
- Broader immunoreactivity due to larger rabbit immune repertoire
Target Information
Histone H3 is a DNA-binding protein found in chromatin of all eukaryotic cells. Together with other core histones, it forms the nucleosome structure. Histones play a central role in transcription regulation, DNA repair, replication, and chromosomal stability.
Post-translational modifications of histone H3, such as acetylation and methylation, influence chromatin structure and gene expression. The N-terminal tail of histone H3 undergoes multiple modifications, including acetylation at lysine residues like K56, which are critical for cellular regulation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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