Thermo Fisher Scientific H3K4ac Recombinant Rabbit Monoclonal Antibody (RM149), ChIP-Verified
H3K4ac에 특이적으로 반응하는 재조합 토끼 단클론 항체로, ChIP 검증 완료. Western blot, ICC/IF, ELISA 등 다양한 응용에 적합. 로트 간 일관성과 높은 특이도 및 감도를 제공하며, 동물 유래 성분이 없는 포뮬레이션.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 0.25–1 µg/mL |
| Immunocytochemistry (ICC/IF) | 0.5–2 µg/mL |
| ELISA | 0.2–1 µg/mL |
| ChIP assay (ChIP) | 1–5 µg |
| Luminex (LUM) | 0.05–0.5 µg/mL |
| Peptide Array (Array) | 0.25 µg/mL |
Product Specifications
| Specification | Detail |
|---|---|
| Species Reactivity | Human, Mouse |
| Published Species | Human |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | RM149 |
| Immunogen | Acetyl-peptide corresponding to the Acetyl-Histone H3 (Lys4) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | PBS, pH 7.2–7.4, with 50% glycerol, 1% BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | −20°C, avoid freeze/thaw cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2866504 |
Product Specific Information
This antibody reacts to Histone H3 acetylated at Lysine 4 (K4ac) and shows no cross-reactivity with other acetylated lysines in histone H3.
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems developed by cloning specific antibody DNA sequences from immunoreactive rabbits. Individual clones are screened to select optimal candidates for production.
Advantages of recombinant rabbit monoclonal antibodies:
- Improved specificity and sensitivity
- Lot-to-lot consistency
- Animal origin-free formulation
- Broad immunoreactivity due to larger rabbit immune repertoire
Click here for Master Lot linking information
Target Information
Histone H3 is a DNA-binding protein found in the chromatin of all eukaryotic cells. Along with other core histones, it forms the nucleosome structure that packages DNA. Histones play a central role in transcription regulation, DNA repair, replication, and chromosomal stability.
The N-terminal tail of histone H3 can undergo multiple post-translational modifications, such as methylation or acetylation of lysine and arginine residues, and phosphorylation of serine or threonine. These modifications influence chromatin structure and gene expression.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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