Thermo Fisher Scientific HLA-DR Monoclonal Antibody (LN3), FITC, eBioscience

Thermo Fisher Scientific HLA-DR Monoclonal Antibody (LN3), FITC, eBioscience

Applications and Tested Dilutions

Product Specifications

Available Conjugate Formats

• Unconjugated
• Alexa Fluor 660, 700, Plus 488, Plus 647
• APC, APC-eFluor 780
• Biotin
• Brilliant UV series (395–805)
• Brilliant Violet series (421–786)
• eFluor 450, 506
• NovaFluor Blue, Violet, UV, Yellow series
• PE, PE-Cyanine5, PE-Cyanine7, PE-eFluor 610
• PerCP-Cyanine5.5
• Super Bright 436, 600, 645, 702, 780
• Custom conjugation available upon request

Product Specific Information

The LN3 monoclonal antibody reacts with human MHC class II molecule HLA-DR, expressed on antigen-presenting cells (APCs) such as B cells, monocytes, macrophages, dendritic cells, and activated T cells.
HLA-DR plays a crucial role in peptide presentation to CD4+ T lymphocytes.

• Applications Reported: Flow cytometric analysis
• Applications Tested: Pre-titrated and tested on normal human peripheral blood cells at 5 µL (0.125 µg) per test
• Excitation: 488 nm; Emission: 520 nm; Laser: Blue
• Filtration: 0.2 µm post-manufacturing filtered

Target Information

HLA-DR is a transmembrane glycoprotein composed of a 36 kDa alpha chain (DRA) and a 27 kDa beta chain (DRB).

• The alpha chain gene (DRA) has 5 exons encoding leader peptide, extracellular domains, transmembrane domain, and cytoplasmic tail.
• DRA acts as the sole alpha chain for DRB1, DRB3, DRB4, and DRB5.
• DRB1 alleles determine peptide binding specificity and are used for transplantation typing.
• Expressed primarily on APCs: B lymphocytes, monocytes, macrophages, thymic epithelial cells, and activated T lymphocytes.
• MHC class II molecules (DR, DQ, DP) bind processed peptides and present them to T-helper cells, initiating immune responses.
• HLA and MHC antibodies are key tools in Immunopeptidomics, enabling neoantigen identification via LC-MS/MS.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

제품 이미지