Thermo Fisher Scientific CD273 (B7-DC) Monoclonal Antibody (122), FITC, eBioscience

Applications and Tested Dilution

• Application: Flow Cytometry (Flow)
• Tested Dilution: 0.5 µg/test
• Publications: View 5 publications

Product Specifications

Available Formats

• Unconjugated
• PE
• PerCP-eFluor 710
• Super Bright 436
• Request custom conjugation

Product Specific Information

Description:
The 122 monoclonal antibody reacts with mouse B7-DC (PD-L2), a member of the B7 family with a molecular weight of ~25 kDa. It is primarily expressed by dendritic cells and monocytes/macrophages in mice. Unlike other B7 family members, B7-DC binds PD-1 rather than CD28/CTLA-4, influencing T cell proliferation depending on activation state. Clone 122 blocks binding of TY25, another anti-mouse B7-DC antibody.

Applications Reported:
Validated for flow cytometric analysis.

Applications Tested:
Tested on mouse bone marrow-derived dendritic cells at ≤0.5 µg/test (100 µL final volume). Optimal titration is recommended.

• Excitation: 488 nm
• Emission: 520 nm
• Laser: Blue Laser
• Filtration: 0.2 µm post-manufacturing filtered

Target Information

Programmed death-ligand 2 (PD-L2, B7-DC):
A B7 ligand family member that binds PD-1, acting as an inhibitory immune checkpoint molecule. Expressed mainly in dendritic cells but inducible in various cell types by cytokines such as IL-4, TNF-α, and IFN-γ. PD-L2 has a higher affinity for PD-1 than PD-L1 and modulates T cell activation and cytokine secretion. It also binds RGMb, influencing respiratory tolerance through BMP/neogenin signaling pathways. The human PD-L2 monomer comprises a 201-aa extracellular domain, 21-aa transmembrane domain, and 32-aa cytoplasmic domain.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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