Thermo Fisher Scientific TCR gamma/delta Monoclonal Antibody (B1.1), FITC, eBioscience

Applications and Tested Dilution

Product Specifications

Additional Formats

• Unconjugated
• Functional Grade
• PE
• PerCP-eFluor 710
• Request custom conjugation

Product Specific Information

Description:
The B1.1 monoclonal antibody reacts with the human gamma delta TCR complex. The gamma delta TCR is expressed by a small subset of T cells in the thymus, peripheral lymphoid tissues, intestinal epithelium, and epidermis. The exact specificity, ligand, and function of gamma delta TCR-bearing T cells are not yet fully understood. It is suggested that these cells recognize bacterial ligands and some tumor cells in the context of MHC class I-like gene products and play a role in immune regulation and bacterial infection response. This monoclonal antibody can be used as a phenotypic marker for gamma delta TCR-expressing T cells.

Applications Reported:
The B1.1 antibody has been reported for use in flow cytometric analysis.

Applications Tested:
Pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. Recommended usage: 5 µL (4 µg) per test (100 µL final volume). Cell count: 10⁵–10⁸ cells/test (empirically determined).

Excitation / Emission: 488 nm / 520 nm
Laser: Blue Laser
Filtration: 0.2 µm post-manufacturing filtered

Target Information

TCR gamma/delta (T-cell receptor gamma/delta) are specialized T-cells in the immune system. TCRs discriminate foreign from self-peptides presented by MHC class II molecules, essential for adaptive immune response. Recognition of self-peptides has been linked to autoimmune diseases, while mutant self-peptides are associated with tumors. Engagement of TCRs by bacterial superantigens can lead to toxic shock syndrome. Autoantibodies to V beta segments of TCRs have been isolated from patients with rheumatoid arthritis (RA) and systemic lupus erythematosus (SLE), potentially modulating inflammatory responses.

Most human T cells express TCR alpha-beta and either CD4 or CD8 (single positive). A small subset lacks both (double negative). Increased alpha-beta DN T cells are found in some autoimmune and immunodeficiency disorders. Gamma-delta T cells are primarily epithelial, showing less TCR diversity and distinct antigen recognition, with subsets exhibiting antitumor and immunoregulatory activity.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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