Thermo Fisher Scientific MCAM (Melanoma Cell Adhesion Molecule)/MUC18/CD146 Recombinant Mouse Monoclonal Antibody (rMUC18, 1130)

Applications

• Immunohistochemistry (Paraffin) (IHC (P)): 1–2 µg/mL

Product Specifications

Product Specific Information

• Immunohistochemistry (PFA fixed): Incubate antibody for 30 minutes at room temperature.
• Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 minutes at 95°C, followed by cooling at room temperature for 20 minutes.

Target Information

CD146 (MCAM) is an integral membrane glycoprotein of the immunoglobulin superfamily. It is expressed at cell-cell junctions in endothelial cells and other normal or malignant cell types.
CD146 functions as a calcium-independent cell adhesion molecule and may act in signal transduction by recruiting Fyn kinase, leading to tyrosine phosphorylation of intracellular proteins involved in actin cytoskeleton assembly.
It plays a role in endothelial cohesion and melanoma metastasis through vascular interaction. Additionally, CD146 is active in neural crest cells during embryonic development and triggers tyrosine phosphorylation of FYN and PTK2, with a transient increase in intracellular calcium concentration.

For Research Use Only.
Not for use in diagnostic procedures or resale without express authorization.

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