Thermo Fisher Scientific NFkB p65 Recombinant Rabbit Monoclonal Antibody (4-2H22L23)
NFkB p65 단백질을 인식하는 재조합 토끼 단클론 항체로, Western blot, IHC, ICC, ChIP 등 다양한 응용에 적합합니다. 높은 특이성과 재현성을 제공하며, Expi293 시스템에서 생산된 동물유래성분 무첨가 항체입니다. 연구용으로만 사용 가능합니다.
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Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1–5 µg/mL | View 2 publications |
| Immunohistochemistry (Paraffin) (IHC-P) | 1:10–1:50 | – |
| Immunocytochemistry (ICC/IF) | 5 µg/mL | View 1 publication |
| ChIP assay (ChIP) | 1–5 µg per 10^6 cells | – |
Product Specifications
| Item | Description |
|---|---|
| Species Reactivity | Human |
| Published Species | Human |
| Host / Isotype | Rabbit / IgG |
| Expression System | Expi293 |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | 4-2H22L23 |
| Immunogen | Recombinant protein corresponding to amino acids 375–551 of NFκB p65 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Protein A |
| Storage Buffer | PBS |
| Contains | 0.09% sodium azide |
| Storage Conditions | Store at 4 °C short term. For long-term storage, store at −20 °C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2532375 |
Product Specific Information
- Intact IgG appears on a non-reducing gel as ~150 kDa band and upon reduction generates ~25 kDa (light chain) and ~50 kDa (heavy chain) bands.
- Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems by cloning antibody DNA sequences from immunoreactive rabbits.
- Each clone is screened to select the best candidate for production.
- Key advantages:
- Superior specificity and sensitivity
- Lot-to-lot consistency
- Animal origin-free formulation
- Broad immunoreactivity due to rabbit immune repertoire
Target Information
Nuclear factor kB p65 (NF-kB p65), encoded by the RELA gene on human chromosome 11, is also known as RelA (v-rel avian reticuloendotheliosis viral oncogene homolog A).
It is one of the two subunits of NF-kB (Nuclear factor kappa-light-chain-enhancer of activated B cells), forming heterodimers with p50 or p52.
Upon TNF-α receptor activation, IKK phosphorylates IκB, leading to its degradation and allowing NF-kB p65 to translocate to the nucleus.
NF-kB p65 plays a critical role in the transcription of the immunoglobulin κ (kappa) gene in mature B-lymphoid cells.
For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.
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