Thermo Fisher Scientific IL-2 Recombinant Rabbit Monoclonal Antibody (2H20L7)
IL-2 단백질을 인식하는 재조합 토끼 단클론 항체로, WB, IHC, Flow, ELISA에 적합합니다. 높은 특이성과 재현성을 제공하며, Protein A로 정제된 액상 형태입니다. 인체 IL-2(aa 21-153)를 면역원으로 제작되었으며, 연구용으로만 사용 가능합니다.
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Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 0.5–2 µg/mL | View 1 publication |
| Immunohistochemistry (Paraffin) (IHC-P) | 1:10–1:50 | - |
| Flow Cytometry (Flow) | 1:100 | - |
| ELISA | 1:50–1:3200 | - |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Published Species | Mouse |
| Host / Isotype | Rabbit / IgG |
| Expression System | Expi293 |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | 2H20L7 |
| Immunogen | Recombinant protein corresponding to amino acids 21–153 of human Interleukin-2 (IL-2; UniProt ID: P60568-1) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Protein A |
| Storage Buffer | PBS |
| Contains | 0.09% sodium azide |
| Storage Conditions | Store at 4°C short term. For long-term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2532376 |
Product Specific Information
- Intact IgG appears as a ~150 kDa band under non-reducing conditions, and under reducing conditions yields ~25 kDa (light chain) and ~50 kDa (heavy chain) bands.
- Clone 701080 successfully detects IL-2 in Flow and ELISA applications.
- Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems by cloning specific antibody DNA sequences from immunoreactive rabbits.
- Advantages include:
- High specificity and sensitivity
- Lot-to-lot consistency
- Animal-origin-free formulation
- Broader immunoreactivity due to the large rabbit immune repertoire
Target Information
Interleukin 2 (IL-2) is an immunomodulatory cytokine crucial for the proliferation of activated T cells, and differentiation of B cells, NK cells, monocytes, and macrophages.
IL-2 signals through the IL-2 receptor (IL-2R), a heterotrimeric complex whose gamma chain is shared with IL-4 and IL-7 receptors.
Expression of the IL-2 gene in mature thymocytes is monoallelic, representing a unique regulatory mechanism for precise gene expression.
Disruption of IL-2–like genes in mice results in ulcerative colitis-like disease, indicating IL-2’s essential role in immune response regulation.
For Research Use Only.
Not for use in diagnostic procedures or resale without express authorization.
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