Thermo Fisher Scientific Symplekin Polyclonal Antibody
Rabbit 폴리클로날 항체로 Human Symplekin(1224–1274) 영역을 인식합니다. Western blot, IHC, IP 등 다양한 응용에 적합하며, 액상 형태로 4°C에서 보관합니다. 연구용으로만 사용되며 FFPE 조직의 IHC 시 citrate buffer(pH 6.0)로 epitope retrieval 권장.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000–1:10,000 |
| Immunohistochemistry (IHC) | 1:100–1:500 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100–1:500 |
| Immunoprecipitation (IP) | 2–5 µg/mg lysate |
Product Specifications
| Property | Description |
|---|---|
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Region between residue 1224 and 1274 of Human Symplekin |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.20 mg/mL |
| Storage Conditions | 4°C |
| Shipping Conditions | Wet ice |
Product Specific Information
- Recommended shelf life: 1 year from date of receipt
- Application Note: For IHC, epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
Target Information
The CD4 antigen is involved in the recognition of MHC class II molecules and acts as a co-receptor for HIV. CD4 is primarily expressed in T helper cells but may also be present in monocytes, macrophages, and dendritic cells.
At the tissue level, CD4 expression can be detected in thymus, lymph nodes, tonsils, spleen, and specific regions of the brain and gut.
CD4 functions to initiate or augment early T-cell activation through association with the T-cell receptor complex and protein tyrosine kinase Lck. It may also mediate neuronal damage in infectious or immune-mediated CNS diseases.
Multiple alternatively spliced transcripts have been identified for this gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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