Thermo Fisher Scientific CD15/FUT4 (Reed-Sternberg Cell Marker) Polyclonal Antibody
CD15/FUT4 폴리클로날 항체로 Reed-Sternberg 세포 마커로 사용됩니다. 인간, 마우스, 랫트 반응성을 가지며 면역조직화학, 면역세포화학, 유세포분석 등에 적합합니다. Protein A로 정제된 액상 형태로, 4°C 보관이 필요합니다.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Immunohistochemistry (Paraffin) (IHC (P)) | Assay-dependent |
| Immunohistochemistry (PFA fixed) (IHC (PFA)) | 5–10 µg/mL |
| Immunocytochemistry (ICC/IF) | 2–4 µg/mL |
| Flow Cytometry (Flow) | 2–4 µg/10^6 cells |
Product Specifications
| Property | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Purified human neutrophils (target: SSEA1 (CD15), UniProt ID: P22083-1, antigen length: 530 aa) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 200 µg/mL |
| Purification | Protein A |
| Storage Buffer | PBS, pH 7.4, with 0.05% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | 4°C, do not freeze |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Target Information
CD15 (Lewis X, Le(x); stage specific embryonic antigen-1, SSEA-1) is a trisaccharide determinant (3-fucosyl-N-acetyllactosamine) expressed on several glycolipids, glycoproteins, and proteoglycans of various cell types such as granulocytes, mast cells, monocytes, macrophages, gastric mucosa cells, nervous system cells, and various tumor cells.
Variants include sialyl-Lewis X and sulphated Lewis X.
Cells with high Le(x) surface expression exhibit strong self-aggregation via calcium-dependent Le(x)-Le(x) interactions, involved in embryo compaction and teratocarcinoma cell autoaggregation.
Sialyl-Le(x) and sialyl-Le(a) act as selectin ligands.
CD15 expression is widely used for confirming Hodgkin’s disease diagnosis.
For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.
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