Thermo Fisher Scientific CXCL11 Polyclonal Antibody, Biotin, PeproTech

Applications

Tested Dilution

Publications

Western Blot (WB)

0.1-0.2 µg/mL

-

ELISA (ELISA)

0.25-1.0 µg/mL

View 1 publication 1 publication

In vitro Assay (IV)

-

View 1 publication 1 publication

Miscellaneous PubMed (Misc)

-

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human

Published species

Human, Mouse

Host/Isotype

Rabbit

Class

Polyclonal

Type

Antibody

Immunogen

E.coli-derived Recombinant Human I-TAC (CXCL11) if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:CXCL11,uniProtId:O14625-1,ncbiNodeId:9606,antigenRange:22-94,antigenLength:94,antigenImageFileName:500-P132BT-25UG_CXCL11_O14625-1_Rabbit.svg,antigenImageFileNamePDP:500-P132BT-25UG_CXCL11_O14625-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Biotin Biotin Biotin

Form

Lyophilized

Concentration

0.1-1.0 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS

Contains

no preservative

Storage conditions

-20°C

Shipping conditions

Ambient

RRID

AB_2929399

Product Specific Information

AA Sequence of recombinant protein: FPMFKRGRCL CIGPGVKAVK VADIEKASIM YPSNNCDKIE VIITLKENKG QRCLNPKSKQ ARLIIKKVER KNF.

Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human I-TAC (CXCL11). Anti-Human I-TAC (CXCL11)-specific antibody was purified by affinity chromatography and then biotinylated.

Sandwich ELISA: To detect Human I-TAC (CXCL11) by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.25-1.0 µg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Human I-TAC (CXCL11) (500-P132) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Human I-TAC (CXCL11).

Western Blot: To detect hI-TAC by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant hI-TAC is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

500-P132BT-1MG will be provided as 2 x 500 µg

Target Information

Interferon-inducible T-cell alpha chemoattractant ( I-TAC), belonging to the CXC chemokine family, is expressed in peripheral blood leukocytes, pancreas and liver, thymus, spleen, lung, small intestine, placenta and prostate. Its molecular weight is 8-11 kD. I-TAC is also known as CXCL-11 and IP-9. The cytokine is involved in regulating calcium release and inducing a chemotactic response in activated T-cells and is the dominant ligand for CXC receptor-3. The gene encoding this protein contains 4 exons and at least three polyadenylation signals which might reflect cell-specific regulation of expression. IFN-gamma is a potent inducer of transcription of this gene. It is upregulated in patients with AIDS, dementia and in patients with multiple sclerosis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.