Thermo Fisher Scientific CXCL11 Polyclonal Antibody, Biotin, PeproTech

Applications and Tested Dilution

Product Specifications

Product Specific Information

Amino Acid Sequence of Recombinant Protein:
FPMFKRGRCL CIGPGVKAVK VADIEKASIM YPSNNCDKIE VIITLKENKG QRCLNPKSKQ ARLIIKKVER KNF

Preparation:
Produced from sera of rabbits immunized with highly pure Recombinant Human I-TAC (CXCL11).
Anti-Human I-TAC (CXCL11)-specific antibody was purified by affinity chromatography and then biotinylated.

Sandwich ELISA:
To detect Human I-TAC (CXCL11) by sandwich ELISA (100 µL/well antibody solution), use 0.25–1.0 µg/mL of this antibody.
In conjunction with PeproTech Polyclonal Anti-Human I-TAC (CXCL11) (500-P132) as a capture antibody, detection of at least 0.2–0.4 ng/well of Recombinant Human I-TAC (CXCL11) is possible.

Western Blot:
To detect hI-TAC by Western Blot, use 0.1–0.2 µg/mL.
Detection limit for Recombinant hI-TAC is 1.5–3.0 ng/lane under reducing or non-reducing conditions.

Package:
500-P132BT-1MG will be provided as 2 × 500 µg.

Target Information

Interferon-inducible T-cell alpha chemoattractant (I-TAC), part of the CXC chemokine family, is expressed in peripheral blood leukocytes, pancreas, liver, thymus, spleen, lung, small intestine, placenta, and prostate.
Its molecular weight is 8–11 kDa. I-TAC is also known as CXCL11 and IP-9.
This cytokine regulates calcium release and induces chemotactic responses in activated T-cells, acting as the dominant ligand for CXC receptor-3.
The gene encoding this protein contains four exons and multiple polyadenylation signals, suggesting cell-specific regulation.
IFN-γ is a potent inducer of this gene’s transcription, and it is upregulated in patients with AIDS, dementia, and multiple sclerosis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.