Thermo Fisher Scientific RANKL (soluble) Polyclonal Antibody, PeproTech
인간 RANKL에 특이적인 토끼 폴리클로날 항체로, Western blot과 ELISA에 최적화되어 있습니다. 항원 친화 크로마토그래피로 정제되었으며, 높은 민감도로 RANKL 단백질 검출이 가능합니다. 연구용으로만 사용됩니다.
- 카탈로그번호
- 500-P133-xxx (3개 옵션)
- 판매단위
- pk
카탈로그
3개 옵션 · 카탈로그 번호를 클릭하면 복사됩니다Thermo Fisher Scientific · Thermo Fisher Scientific RANKL (soluble) Polyclonal Antibody, PeproTech
Thermo Fisher Scientific RANKL (soluble) Polyclonal Antibody, PeproTech
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 0.1–0.2 µg/mL | View 2 publications |
| ELISA | 0.5–2.0 µg/mL | View 2 publications |
| In vitro Assay (IV) | – | View 2 publications |
| Immunostaining (IS) | – | View 1 publication |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Published Species | Human, Mouse |
| Host / Isotype | Rabbit |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | E.coli-derived Recombinant Human sRANK Ligand |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 0.1–1.0 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS |
| Contains | No preservative |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient |
| RRID | AB_2929401 |
Product Specific Information
AA Sequence of recombinant protein:
MEKAMVDGSW LDLAKRSKLE AQPFAHLTIN ATDIPSGSHK VSLSSWYHDR GWAKISNMTF SNGKLIVNQD GFYYLYANIC FRHHETSGDL ATEYLQLMVY VTKTSIKIPS SHTLMKGGST KYWSGNSEFH FYSINVGGFF KLRSGEEISI EVSNPSLLDP DQDATYFGAF KVRDID
Preparation:
Produced from sera of rabbits immunized with highly pure Recombinant Human sRANK Ligand. Anti-Human sRANK Ligand-specific antibody was purified by affinity chromatography employing an immobilized Human sRANK Ligand matrix.
Sandwich ELISA:
To detect Human sRANK Ligand by sandwich ELISA (using 100 µL/well antibody solution), a concentration of 0.5–2.0 µg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech Biotinylated Anti-Human sRANK Ligand (500-P133Bt) as a detection antibody, allows the detection of at least 0.2–0.4 ng/well of Recombinant Human sRANK Ligand.
Western Blot:
To detect Human sRANK Ligand by Western Blot analysis, this antibody can be used at a concentration of 0.1–0.2 µg/mL. Used in conjunction with compatible secondary reagents, the detection limit for Recombinant Human sRANK Ligand is 1.5–3.0 ng/lane under either reducing or non-reducing conditions.
Target Information
This gene encodes a member of the tumor necrosis factor (TNF) cytokine family which is a ligand for osteoprotegerin and functions as a key factor for osteoclast differentiation and activation. It acts as a dendritic cell survival factor and regulates T cell-dependent immune responses. T cell activation induces expression of this gene, leading to increased osteoclastogenesis and bone loss.
The protein activates antiapoptotic kinase AKT/PKB through a signaling complex involving SRC kinase and TRAF6, suggesting a role in apoptosis regulation.
Targeted disruption of the related gene in mice results in severe osteopetrosis and lack of osteoclasts, with defects in T and B lymphocyte differentiation and mammary structure formation during pregnancy. Two alternatively spliced transcript variants have been identified.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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