Thermo Fisher Scientific RANKL (soluble) Polyclonal Antibody, PeproTech

Thermo Fisher Scientific RANKL (soluble) Polyclonal Antibody, PeproTech

Applications and Tested Dilution

Product Specifications

Product Specific Information

AA Sequence of recombinant protein:
MEKAMVDGSW LDLAKRSKLE AQPFAHLTIN ATDIPSGSHK VSLSSWYHDR GWAKISNMTF SNGKLIVNQD GFYYLYANIC FRHHETSGDL ATEYLQLMVY VTKTSIKIPS SHTLMKGGST KYWSGNSEFH FYSINVGGFF KLRSGEEISI EVSNPSLLDP DQDATYFGAF KVRDID

Preparation:
Produced from sera of rabbits immunized with highly pure Recombinant Human sRANK Ligand. Anti-Human sRANK Ligand-specific antibody was purified by affinity chromatography employing an immobilized Human sRANK Ligand matrix.

Sandwich ELISA:
To detect Human sRANK Ligand by sandwich ELISA (using 100 µL/well antibody solution), a concentration of 0.5–2.0 µg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech Biotinylated Anti-Human sRANK Ligand (500-P133Bt) as a detection antibody, allows the detection of at least 0.2–0.4 ng/well of Recombinant Human sRANK Ligand.

Western Blot:
To detect Human sRANK Ligand by Western Blot analysis, this antibody can be used at a concentration of 0.1–0.2 µg/mL. Used in conjunction with compatible secondary reagents, the detection limit for Recombinant Human sRANK Ligand is 1.5–3.0 ng/lane under either reducing or non-reducing conditions.

Target Information

This gene encodes a member of the tumor necrosis factor (TNF) cytokine family which is a ligand for osteoprotegerin and functions as a key factor for osteoclast differentiation and activation. It acts as a dendritic cell survival factor and regulates T cell-dependent immune responses. T cell activation induces expression of this gene, leading to increased osteoclastogenesis and bone loss.
The protein activates antiapoptotic kinase AKT/PKB through a signaling complex involving SRC kinase and TRAF6, suggesting a role in apoptosis regulation.
Targeted disruption of the related gene in mice results in severe osteopetrosis and lack of osteoclasts, with defects in T and B lymphocyte differentiation and mammary structure formation during pregnancy. Two alternatively spliced transcript variants have been identified.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.