Thermo Fisher Scientific CD140b (PDGFRB) Monoclonal Antibody (APB5), Alexa Fluor 488, eBioscience
마우스 CD140b(PDGFRB) 인식 단일클론 항체로, Alexa Fluor 488로 표지되어 형광 분석에 적합합니다. Flow cytometry에 최적화되어 있으며, 488 nm 레이저에서 519 nm 방출 특성을 가집니다. 4°C 암소 보관, 동결 금지.
- 카탈로그번호
- 53-1402-82
- 판매단위
- pk
카탈로그
1개 옵션 · 카탈로그 번호를 클릭하면 복사됩니다Thermo Fisher Scientific · Thermo Fisher Scientific CD140b (PDGFRB) Monoclonal Antibody (APB5), Alexa Fluor 488, eBioscience
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Immunohistochemistry (IHC) | - | View 6 publications |
| Immunohistochemistry (Frozen) (IHC (F)) | - | View 1 publication |
| Immunocytochemistry (ICC/IF) | - | View 7 publications |
| Flow Cytometry (Flow) | 1.0 µg/test | View 7 publications |
| Neutralization (Neu) | - | View 1 publication |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Mouse |
| Published Species | Not Applicable |
| Host / Isotype | Rat / IgG2a, kappa |
| Recommended Isotype Control | Rat IgG2a kappa Isotype Control (eBR2a), Alexa Fluor™ 488, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | APB5 |
| Conjugate | Alexa Fluor™ 488 |
| Excitation / Emission Max | 499 / 520 nm |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2815208 |
Available Formats
- Unconjugated
- APC
- APC-eFluor 780
- Biotin
- Functional Grade
- PE
- PE-Cyanine7
- PE-eFluor 610
- Super Bright 436
- Super Bright 600
- Super Bright 780
- Request custom conjugation
Product Specific Information
The APB5 monoclonal antibody reacts with the mouse CD140b molecule, the beta chain of the platelet-derived growth factor receptor (PDGF receptor). PDGFRβ is a receptor tyrosine kinase that forms dimers upon ligand binding and phosphorylates substrates. Dimers may be alpha/alpha, beta/beta, or alpha/beta heterodimers, serving as substrates for kinase activity. CD140b is expressed in embryonic tissues and mesenchymal-derived adult mouse cells. PDGFRβ plays a significant role in the formation of fibrous atherosclerotic lesions.
Applications Reported: Flow cytometric analysis.
Applications Tested: Tested by flow cytometry on NIH/3T3 cells. Recommended ≤1.0 µg/test (100 µL final volume). Cell number: 10⁵–10⁸ cells/test. Optimize concentration for best performance.
Excitation: 488 nm
Emission: 519 nm
Laser: Blue Laser
Target Information
PDGFRB is a receptor tyrosine kinase implicated in atherogenesis and cell transformation by the SIS oncogene. These growth factors act as mitogens for mesenchymal-origin cells. Ligand binding determines receptor dimerization (homodimer or heterodimer of PDGFRα/β). The gene is located on chromosome 5, near GM-CSF and M-CSF receptor genes, possibly linked to the 5-q syndrome. A translocation between chromosomes 5 and 12 fusing PDGFRB and ETV6 results in chronic myeloproliferative disorder with eosinophilia.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
제품 이미지
Thermo Fisher Scientific 상품 둘러보기
전체보기
문의
0개 · 배송·재고 문의는 실시간 상담이 빠릅니다아직 등록된 문의가 없어요.