Thermo Fisher Scientific CD140b (PDGFRB) Monoclonal Antibody (APB5), Alexa Fluor 488, eBioscience

Applications and Tested Dilution

Product Specifications

Available Formats

• Unconjugated
• APC
• APC-eFluor 780
• Biotin
• Functional Grade
• PE
• PE-Cyanine7
• PE-eFluor 610
• Super Bright 436
• Super Bright 600
• Super Bright 780
• Request custom conjugation

Product Specific Information

The APB5 monoclonal antibody reacts with the mouse CD140b molecule, the beta chain of the platelet-derived growth factor receptor (PDGF receptor). PDGFRβ is a receptor tyrosine kinase that forms dimers upon ligand binding and phosphorylates substrates. Dimers may be alpha/alpha, beta/beta, or alpha/beta heterodimers, serving as substrates for kinase activity. CD140b is expressed in embryonic tissues and mesenchymal-derived adult mouse cells. PDGFRβ plays a significant role in the formation of fibrous atherosclerotic lesions.

Applications Reported: Flow cytometric analysis.
Applications Tested: Tested by flow cytometry on NIH/3T3 cells. Recommended ≤1.0 µg/test (100 µL final volume). Cell number: 10⁵–10⁸ cells/test. Optimize concentration for best performance.
Excitation: 488 nm
Emission: 519 nm
Laser: Blue Laser

Target Information

PDGFRB is a receptor tyrosine kinase implicated in atherogenesis and cell transformation by the SIS oncogene. These growth factors act as mitogens for mesenchymal-origin cells. Ligand binding determines receptor dimerization (homodimer or heterodimer of PDGFRα/β). The gene is located on chromosome 5, near GM-CSF and M-CSF receptor genes, possibly linked to the 5-q syndrome. A translocation between chromosomes 5 and 12 fusing PDGFRB and ETV6 results in chronic myeloproliferative disorder with eosinophilia.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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