Thermo Fisher Scientific CD144 (VE-cadherin) Monoclonal Antibody (eBioBV13 (BV13)), Alexa Fluor 488, eBioscience

Applications

Tested Dilution

Publications

Western Blot (WB)

-

View 3 publications 3 publications

Immunohistochemistry (IHC)

-

View 7 publications 7 publications

Immunohistochemistry (Frozen) (IHC (F))

Assay-Dependent

-

Immunocytochemistry (ICC/IF)

-

View 7 publications 7 publications

Flow Cytometry (Flow)

0.5 µg/test

View 9 publications 9 publications

ChIP assay (ChIP)

-

View 1 publication 1 publication

Peptide Array (Array)

-

View 1 publication 1 publication

Product Specifications

Published species

Human, Mouse

Host/Isotype

Rat / IgG1, kappa

Recommended Isotype Control

Rat IgG1 kappa Isotype Control (eBRG1), Alexa Fluor™ 488, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

eBioBV13 (BV13)

Conjugate

Alexa Fluor™ 488 Alexa Fluor™ 488 Alexa Fluor™ 488

View additional formats

• Unconjugated
• APC
• Biotin
• eFluor 450
• eFluor 660
• Functional Grade
• PE
• PE-Cyanine7
• PerCP-eFluor 710
• Super Bright 600
• Request custom conjugation

Excitation/Emission Max

499/520 nm View spectra

Form

Liquid

Concentration

0.5 mg/mL

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Wet ice

RRID

AB_1210528

Product Specific Information

Description: The BV13 monoclonal antibody reacts with mouse VE-Cadherin (CD144). VE-Cadherin is a 120 kDa member of the type II Cadherin family, characterized by the presence of 5 extracellular cadherin domains (ECD), and anchored to the actin cytoskeleton through their cytoplasmic tail. VE-Cadherin mediates homophilic adhesion between neighbouring endothelial cells and is localized within specialized structures at cell-cell contacts, called adherens junctions. VE-Cadherin is expressed constitutively throughout the entire vasculature, and is required for numerous endothelial cell functions including migration, survival, contact-dependent growth inhibition and endothelial cell assembly into tubular structures. Furthermore, it is thought that VE-Cadherin+CD45- cells from the yolk sac or aorta-gonad-mesonephros (AGM) have the potential to give rise to hematopoietic cells.

Applications Reported: This eBioBV13 (BV13) antibody has been reported for use in flow cytometric analysis, and immunohistology staining of frozen tissue sections.

Applications Tested: This eBioBV13 (BV13) antibody has been tested by flow cytometric analysis of bEnd.3 cells. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Excitation: 488 nm; Emission: 519 nm; Laser: Blue Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

VE-cadherin is a member of the cadherin superfamily that is located in a six-cadherin cluster in a region on the long arm of chromosome 16 and is involved in loss of heterozygosity events in breast and prostate cancer. VE-cadherin protein is a calcium-dependent cell-cell adhesion glycoprotein comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. Functioning as a classic cadherin by imparting to cells the ability to adhere in a homophilic manner, VE-cadherin may play an important role in endothelial cell biology through control of the cohesion and organization of the intercellular junctions. An alternative splice variant has been described but the full length sequence of VE-cadherin has not been determined.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.