Thermo Fisher Scientific ATGL Monoclonal Antibody (K.751.7)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

View 5 publications 5 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:50

-

Immunocytochemistry (ICC/IF)

1:100

-

Immunoprecipitation (IP)

1:50

View 1 publication 1 publication

Product Specifications

Species Reactivity

Mouse, Rat

Published species

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Monoclonal

Type

Antibody

Clone

K.751.7

Immunogen

Synthetic peptide corresponding to residues surrounding Pro469 of mouse ATGL if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:ATGL,uniProtId:Q8BJ56-1,ncbiNodeId:10090,antigenRange:469,antigenLength:486,antigenImageFileName:MA5-14990_ATGL_Q8BJ56-1_Rabbit.svg,antigenImageFileNamePDP:MA5-14990_ATGL_Q8BJ56-1_Rabbit_PDP.jpeg,sortOrder:2}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

23 µg/mL

Purification

Affinity chromatography

Storage buffer

0.01M HEPES, pH 7.5, with 0.15M NaCl, 100µg/mL BSA, 50% glycerol

Contains

<0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice

RRID

AB_10974333

Product Specific Information

It is not recommended to aliquot this antibody.

Target Information

Lipolytic enzymes are required for mobilization of fatty acids from triglyceride stores in adipose tissue. Energy homeostasis is affected by dysfunctional lipolysis and may contribute to the pathogenesis of obesity and insulin resistance. Until recently, hormone-sensitive lipase (HSL) was the only enzyme known to hydrolyze triglycerides in mammalian adipose tissue. It is now thought that a second enzyme, adipose triglyceride lipase (ATGL), catalyzes the initial step in triglyceride hydrolysis. ATGL is highly expressed in adipose tissue of mice and humans. It exhibits high substrate specificity for triacylglycerol and is associated with lipid droplets. Inhibition of ATGL markedly decreases total adipose acyl-hydrolase activity. Thus, ATGL and HSL coordinately catabolize stored triglycerides in adipose tissue of mammals.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.