Thermo Fisher Scientific ATGL Monoclonal Antibody (K.751.7)
ATGL 단백질을 검출하기 위한 Rabbit monoclonal 항체로, Western blot, IHC, ICC, IP 등 다양한 응용에 적합합니다. Mouse 및 Rat 시료에 반응하며, 고순도의 액상 형태로 제공됩니다. 지방 분해 효소 연구 및 대사 관련 연구에 유용합니다.
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Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:1,000 | View 5 publications |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50 | - |
| Immunocytochemistry (ICC/IF) | 1:100 | - |
| Immunoprecipitation (IP) | 1:50 | View 1 publication |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Mouse, Rat |
| Published Species | Human, Mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Monoclonal |
| Type | Antibody |
| Clone | K.751.7 |
| Immunogen | Synthetic peptide corresponding to residues surrounding Pro469 of mouse ATGL |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 23 µg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | 0.01M HEPES, pH 7.5, with 0.15M NaCl, 100 µg/mL BSA, 50% glycerol |
| Contains | <0.02% sodium azide |
| Storage Conditions | -20°C |
| Shipping Conditions | Wet ice |
| RRID | AB_10974333 |
Product Specific Information
It is not recommended to aliquot this antibody.
Target Information
Lipolytic enzymes are required for mobilization of fatty acids from triglyceride stores in adipose tissue. Energy homeostasis is affected by dysfunctional lipolysis and may contribute to the pathogenesis of obesity and insulin resistance.
Until recently, hormone-sensitive lipase (HSL) was the only enzyme known to hydrolyze triglycerides in mammalian adipose tissue. It is now thought that a second enzyme, adipose triglyceride lipase (ATGL), catalyzes the initial step in triglyceride hydrolysis.
ATGL is highly expressed in adipose tissue of mice and humans. It exhibits high substrate specificity for triacylglycerol and is associated with lipid droplets. Inhibition of ATGL markedly decreases total adipose acyl-hydrolase activity. Thus, ATGL and HSL coordinately catabolize stored triglycerides in adipose tissue of mammals.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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