Thermo Fisher Scientific NFAT5 Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

View 25 publications 25 publications

Immunohistochemistry (IHC)

-

View 3 publications 3 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:20

-

Immunocytochemistry (ICC/IF)

1:1,000

View 2 publications 2 publications

Immunoprecipitation (IP)

3 µg

-

ChIP assay (ChIP)

-

View 2 publications 2 publications

Gel Shift (GS)

Assay-dependent

-

Product Specifications

Species Reactivity

Human, Mouse

Published species

Human, Mouse, Rabbit, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptide corresponding to residues C D(1439) L L V S L Q N Q G N N L T G S F(1455) of human NFAT5. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:NFAT5,uniProtId:O94916-1,ncbiNodeId:9606,antigenRange:1439-1455,antigenLength:1531,antigenImageFileName:PA1-023_NFAT5_O94916-1_Rabbit.svg,antigenImageFileNamePDP:PA1-023_NFAT5_O94916-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS with 1mg/mL BSA

Contains

0.05% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2152617

Product Specific Information

PA1-023 detects nuclear factor of activated T-cells 5 (NFAT5) from human and mouse cells as well as recombinant human protein.

PA1-023 has been successfully used in Western blot, immunofluorescence, immunoprecipitation, gel shift and immunocytochemistry procedures. By Western blot, this antibody detects an ~170 kDa protein representing NFAT5 in HEK293 cells transfected with the human NFAT5 gene. Immunocytochemical staining of NFAT5 in HEK293 cells transfected with the human NFAT5 gene with PA1-023 results in primarily cytoplasmic staining.

The PA1-023 immunogen is a synthetic peptide corresponding to residues C D(1439) L L V S L Q N Q G N N L T G S F(1455) of human NFAT5. This sequence is 94% conserved in the mouse protein. PA1-023 immunizing peptide (Cat. # PEP-123) is available for use in neutralization and control experiments.

Target Information

The nuclear factor of activated T-cells (NFAT) transcription complex is required for the expression of a group of proteins that collectively regulate the immune response. Four NFAT proteins, encoded on separate genes and expressed as several splice variants, have been described: NFAT1 (also known as NFATp or NFATc2), NFAT2 (NFATc or NFATc1), NFAT3, and NFAT4 (NFATx or NFATc3). These proteins show a low level of sequence similarity with the Dorsal/Rel/NFkB family of transcription factors. Another NFAT-related protein termed NFAT5 differs from isoforms 1-4 in that it lacks many of the Fos/Jun contact sites observed in its predecessors and its subcellular localization is not calcineurin-dependent.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.