Thermo Fisher Scientific NFAT5 Polyclonal Antibody

Applications and Tested Dilutions

Product Specifications

Product Specific Information

PA1-023 detects nuclear factor of activated T-cells 5 (NFAT5) from human and mouse cells as well as recombinant human protein.
This antibody has been successfully used in Western blot, immunofluorescence, immunoprecipitation, gel shift, and immunocytochemistry procedures.
By Western blot, it detects an ~170 kDa protein representing NFAT5 in HEK293 cells transfected with the human NFAT5 gene.
Immunocytochemical staining of NFAT5 in HEK293 cells transfected with the human NFAT5 gene with PA1-023 results in primarily cytoplasmic staining.

The immunogen is a synthetic peptide corresponding to residues C D(1439) L L V S L Q N Q G N N L T G S F(1455) of human NFAT5. This sequence is 94% conserved in the mouse protein.
The PA1-023 immunizing peptide (Cat. # PEP-123) is available for use in neutralization and control experiments.

Target Information

The nuclear factor of activated T-cells (NFAT) transcription complex is required for the expression of a group of proteins that collectively regulate the immune response.
Four NFAT proteins—NFAT1 (NFATp or NFATc2), NFAT2 (NFATc or NFATc1), NFAT3, and NFAT4 (NFATx or NFATc3)—have been described, each encoded on separate genes and expressed as several splice variants.
These proteins show low sequence similarity with the Dorsal/Rel/NFkB family of transcription factors.
NFAT5 differs from isoforms 1–4 in that it lacks many of the Fos/Jun contact sites observed in its predecessors and its subcellular localization is not calcineurin-dependent.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.