Thermo Fisher Scientific Phospho-HSP27 (Ser15) Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

View 4 publications 4 publications

Immunocytochemistry (ICC/IF)

1:50-1:200

-

Immunoprecipitation (IP)

3 µg

-

Product Specifications

Species Reactivity

Human, Rat

Published species

Rabbit, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic phosphopeptide corresponding to residues L(10) L R G P (pS) W D P F R C(21) of human HSP27. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:HSP27,uniProtId:P04792-1,ncbiNodeId:9606,antigenRange:10-21,antigenLength:205,antigenImageFileName:PA1-016_HSP27_P04792-1_Rabbit.svg,antigenImageFileNamePDP:PA1-016_HSP27_P04792-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS with 1mg/mL BSA

Contains

0.05% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2120943

Product Specific Information

PA1-016 detects phosphorylated heat shock protein 27 (hsp27) from rat and human tissue tissues.

PA1-016 has been successfully used in Western blot, immunoprecipitation, and immunofluorescence procedures. By Western blot, this antibody detects a ~27 kDa protein which corresponds to phospho-hsp27 (Ser15) from HeLa cells. For immunolocalization, Cat. # PA1-018 is recommended.

The PA1-016 immunogen is a synthetic phosphopeptide corresponding to residues L(10) L R G P (pS) W D P F R C(21) of human HSP27. The immunizing peptide is 81% conserved in mice, rats, and hamsters. The phosphorylated peptide (Cat. # PEP-187) and non-phosphorylated peptide (Cat. # PEP-240) are available for use in neutralization and control experiments.

Target Information

In response to adverse changes in their environment, cells from many organisms increase the expression of a class of proteins referred to as heat shock or stress proteins. HSPB1 (heat shock protein beta-1 or HSP27) is a small heat shock protein which functions as a molecular chaperone that maintains denatured proteins in a folding-competent state. It plays a role in stress resistance and actin organization. Through its molecular chaperone activity, HSP27 regulates numerous biological processes including the phosphorylation and the axonal transport of neurofilament proteins. Mutations in the gene can result in Charcot-Marie-Tooth disease 2F and Neuronopathy distal hereditary motor 2B.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.