Thermo Fisher Scientific Phospho-HSP27 (Ser15) Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:100-1:1,000

View 2 publications 2 publications

Immunohistochemistry (IHC)

1:50 - 1:200

View 1 publication 1 publication

Immunohistochemistry (Frozen) (IHC (F))

-

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

1:50 - 1:200

View 3 publications 3 publications

Immunoprecipitation (IP)

3 µg

-

Product Specifications

Species Reactivity

Human, Rat

Published species

Human, Mouse, Rabbit, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic phosphopeptide corresponding to the residues L(10) L R G P (pS) W D P F R C(21) of human HSP27. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:HSP27,uniProtId:P04792-1,ncbiNodeId:9606,antigenRange:10-21,antigenLength:205,antigenImageFileName:PA1-018_HSP27_P04792-1_Rabbit.svg,antigenImageFileNamePDP:PA1-018_HSP27_P04792-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS with 1mg/mL BSA

Contains

0.05% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_325019

Product Specific Information

PA1-018 detects phosphorylated heat shock protein 27(hsp27) from rat and human tissue tissues.

PA1-018 has been successfully used in Western blot, immunoprecipitation, immunofluorscence, immunocytochemistry and immunohistochemistry procedures. Immunocytochemical staining of phospho-hsp27 (Ser15) in HeLa cells with this antibody results in cytoplasmic staining.

The PA1-018 immunogen is a synthetic phosphopeptide corresponding to the residues L(10) L R G P (pS) W D P F R C(21) of human HSP27. The immunizing peptide is 81% homologous in mice, rats, and hamsters. This peptide (Cat. # PEP-187) is available for use in neutralization and control experiments.

Target Information

In response to adverse changes in their environment, cells from many organisms increase the expression of a class of proteins referred to as heat shock or stress proteins. HSPB1 (heat shock protein beta-1 or HSP27) is a small heat shock protein which functions as a molecular chaperone that maintains denatured proteins in a folding-competent state. It plays a role in stress resistance and actin organization. Through its molecular chaperone activity, HSP27 regulates numerous biological processes including the phosphorylation and the axonal transport of neurofilament proteins. Mutations in the gene can result in Charcot-Marie-Tooth disease 2F and Neuronopathy distal hereditary motor 2B.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.