Thermo Fisher Scientific CRP Monoclonal Antibody (1G1)
CRP 단백질을 검출하는 Thermo Fisher Scientific의 Mouse Monoclonal Antibody (Clone 1G1). Western blot, IHC, ICC/IF, Flow cytometry 및 ELISA에 적합. 인간 시료에서 높은 특이성과 재현성을 제공하며, 연구용으로 사용.
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Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:500–1:2,000 | – |
| Immunohistochemistry (Paraffin) (IHC-P) | 1:200–1:1,000 | – |
| Immunohistochemistry (Frozen) (IHC-F) | – | 1 publication |
| Immunocytochemistry (ICC/IF) | 1:200 | – |
| Flow Cytometry (Flow) | 1:200–1:400 | 1 publication |
| ELISA | 1:10,000 | – |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Published Species | Human, Mouse |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 1G1 |
| Immunogen | Purified recombinant fragment of human CRP expressed in E. coli |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | Not determined |
| Storage Buffer | Ascites |
| Contains | 0.03% sodium azide |
| Storage Conditions | Store at 4°C short term; for long-term, store at −20°C avoiding freeze/thaw cycles |
| Shipping Conditions | Wet ice |
| RRID | AB_2538532 |
Product Specific Information
MA5-17061 targets CRP in FACS, IHC, indirect ELISA, and WB applications and shows reactivity with human samples.
The immunogen is a purified recombinant fragment of human CRP expressed in E. coli.
MA5-17061 detects CRP with a predicted molecular weight of approximately 25 kDa.
Target Information
CRP (C-reactive protein) is a major cyclic, pentameric acute-phase protein composed of five identical, noncovalently bound, nonglycosylated subunits. Each subunit consists of 206 amino acids and has a molecular weight of 24 kDa. CRP is produced by the liver, and plasma levels rise dramatically during inflammatory processes.
CRP initiates the classical complement cascade, binds nuclear components (chromatin, histones, etc.), and plays a key role in innate immunity and nonspecific host resistance. Levels can increase 100–1000 fold in response to injury, infection, or inflammation.
It functions as an opsonin, complement activator, and monocyte/macrophage activator. CRP also binds chromatin, histones, and snRNP, suggesting a scavenger role during cell necrosis. Elevated basal CRP levels are associated with increased risk of hypertension and cardiovascular disease.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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