Thermo Fisher Scientific GAPex5 Polyclonal Antibody

Applications

Western Blot (WB)

• Tested Dilution: 1:2,000–1:10,000

Immunoprecipitation (IP)

• Tested Dilution: 2–5 µg/mg lysate

Product Specifications

Product Specific Information

• Recommended shelf life: 1 year from date of receipt
• Based on 100% sequence identity, this antibody is predicted to react with Mouse and Bovine.

Target Information

GAPVD1 acts both as a GTPase-activating protein (GAP) and a guanine nucleotide exchange factor (GEF), participating in processes such as endocytosis, insulin receptor internalization, and LC2A4/GLUT4 trafficking.
It acts as a GEF for the Ras-related protein RAB31 by exchanging bound GDP for free GTP, regulating LC2A4/GLUT4 trafficking.
In the absence of insulin, it maintains RAB31 in an active state and promotes a futile cycle between LC2A4/GLUT4 storage vesicles and early endosomes, retaining LC2A4/GLUT4 inside the cells.
Upon insulin stimulation, it translocates to the plasma membrane, releasing LC2A4/GLUT4 from intracellular storage vesicles.
It is also involved in EGFR trafficking and degradation, possibly by promoting EGFR ubiquitination and subsequent degradation by the proteasome.
It has GEF activity for Rab5 and GAP activity for Ras.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

제품 이미지

(이미지 파일: A302-116A-M_GAPVD1_Q14C86-1_Rabbit.svg, A302-116A-M_GAPVD1_Q14C86-1_Rabbit_PDP.jpeg)