Thermo Fisher Scientific CD105 Monoclonal Antibody (MEM-229)
CD105 (Endoglin)을 인식하는 Mouse monoclonal antibody MEM-229 클론. 인간 및 돼지 반응성, 다양한 응용(WB, IHC, ICC/IF, Flow)에 사용 가능. 액상형, Protein A 정제, 4°C 보관. 연구용으로만 사용.
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Thermo Fisher Scientific CD105 Monoclonal Antibody (MEM-229)
Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | Assay-dependent |
| Immunohistochemistry (Frozen) (IHC (F)) | 1:200 |
| Immunocytochemistry (ICC/IF) | Assay-dependent |
| Flow Cytometry (Flow) | 2–6 µg/mL |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Pig |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Clone | MEM-229 |
| Immunogen | Recombinant Vaccinia virus containing human CD105 (L-isoform) cDNA |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | PBS, pH 7.4 |
| Contains | 15 mM sodium azide |
| Storage Conditions | 4°C, do not freeze |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_1071122 |
Additional Formats
Product Specific Information
This antibody recognizes an extracellular epitope of CD105 (Endoglin), a 90 kDa type I integral membrane homodimer glycoprotein expressed on vascular endothelial cells (small and large vessels), activated monocytes and tissue macrophages, stromal cells of certain tissues including bone marrow, pre-B lymphocytes in fetal marrow and erythroid precursors in fetal and adult bone marrow. It is also present on syncytiotrophoblast on placenta throughout pregnancy.
Western Blot: non-reducing conditions; Immunohistochemistry (Frozen): acetone fixation.
Target Information
CD105 (Endoglin) is a Type I transmembrane protein highly expressed on human vascular endothelial cells. It exists as an O- and N-glycosylated homodimer. Upregulation of endoglin expression has been demonstrated in tumor vasculature and proliferating cells, suggesting it is a proliferation-associated endothelial marker. CD105 binds TGF beta 1 and 3 with high affinity, but not TGF beta 2.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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