Thermo Fisher Scientific alpha/beta Tubulin Monoclonal Antibody (TU-10)

Applications

Tested Dilution

Publications

Western Blot (WB)

2 µg/mL

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

Assay-dependent

View 1 publication 1 publication

Flow Cytometry (Flow)

8-12 µg/mL

-

Product Specifications

Species Reactivity

Human, Many, Mouse, Pig, Rat

Published species

Human

Host/Isotype

Mouse / IgM

Class

Monoclonal

Type

Antibody

Clone

TU-10

Immunogen

Microtubule proteins from porcine brain. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:alpha/beta Tubulin,uniProtId:P02550-1,ncbiNodeId:9821,antigenRange:1-451,antigenLength:451,antigenImageFileName:MA1-19400_alphabeta_Tubulin_P02550-1_House_mouse.svg,antigenImageFileNamePDP:MA1-19400_alphabeta_Tubulin_P02550-1_House_mouse_PDP.jpeg,sortOrder:1},{targetFamily:alpha/beta Tubulin,uniProtId:Q767L7-1,ncbiNodeId:9821,antigenRange:1-444,antigenLength:444,antigenImageFileName:MA1-19400_alphabeta_Tubulin_Q767L7-1_House_mouse.svg,antigenImageFileNamePDP:MA1-19400_alphabeta_Tubulin_Q767L7-1_House_mouse_PDP.jpeg,sortOrder:2}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

View additional formats

• Alexa Fluor 488
• Request custom conjugation

Form

Liquid

Concentration

1 mg/mL

Purification

purified

Storage buffer

TBS, pH 8

Contains

15mM sodium azide

Storage conditions

4° C, do not freeze

Shipping conditions

Wet ice

RRID

AB_1070282

Product Specific Information

This antibody recognizes alpha, beta-tubulin heterodimer, a basic intracellular structural unit of microtubules. Alpha- and beta-tubulins form approximately 100 kDa tubulin heterodimer, a globular protein that polymerizes to form microtubules.

Western Blot: Reducing conditions.

Target Information

Tubulin protein is the major constituent of microtubules. Tubulin is a dimer of alpha and beta chains, which binds two molecules of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha-chain. There are at least six isotypes of both alpha- and beta- tubulin in human cells, which are distinguished by slightly different amino acid sequences and encoded by a large, multigene family that has been highly conserved throughout evolution. Although the most important functions of microtubules in proliferative cells are through their actions as components of the mitotic spindle, they are also involved in many other essential functions throughout the cell cycle of both malignant and nonmalignant cells. Anti-microtubule agents including Vinca alkaloids and taxanes may disrupt many of these essential functions.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.