Thermo Fisher Scientific SEMA3B Polyclonal Antibody
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Western blot, IHC, ICC에 적합한 SEMA3B 폴리클로날 항체. Rabbit IgG로 제작되었으며, 인간 및 다양한 종에 반응. 항원 친화 크로마토그래피로 정제된 고품질 항체로, 연구용으로 신경 발달 및 종양 억제 연구에 활용 가능.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:5,000–1:20,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100–1:200 |
| Immunocytochemistry (ICC/IF) | 1:40 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Bovine, Dog, Human, Mouse, Non-human primate, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide between amino acids 100–200 of the human SEMA3B protein |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | TBS |
| Contains | 0.05% sodium azide |
| Storage Conditions | −20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Wet ice |
| RRID | AB_2185418 |
Product Specific Information
- In Western Blot, a band is seen at approximately 50 kDa (secreted form) and a faint band at approximately 83 kDa (pro-form).
- For immunostaining of paraffin-embedded tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended.
- Suggested positive controls: antigen standard for SEMA3B (transient overexpression lysate), mouse cerebellum protein, mouse E16 cerebellum lysate.
Target Information
The semaphorin/collapsin family of molecules plays a critical role in guiding growth cones during neuronal development. The secreted protein encoded by this gene is important in axonal guidance and has been shown to act as a tumor suppressor by inducing apoptosis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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