Thermo Fisher Scientific HMGB1 Polyclonal Antibody

Applications and Tested Dilution

Product Specifications

Product Specific Information

This antibody is expected to cross-react with porcine, equine, and rabbit samples based on 100% sequence homology.
Suggested positive control: HeLa whole cell extract, antigen standard for HMGB1 (transient overexpression lysate).

Target Information

HMGB1 (High-mobility group box-1) is a nuclear non-histone DNA-binding chromosomal protein. It can be released from damaged or necrotic cells and acts as a proinflammatory cytokine.
Mouse HMGB1 is a 215 amino acid single-chain polypeptide containing three domains:

• Two DNA-binding domains (HMG box A: aa 9–79; box B: aa 89–162)
• A negatively charged C-terminal acidic tail (aa 186–215)

Residues 28–44 and 180–185 contain nuclear localization signals (NLS).
The B box domain mediates cytokine activity, while the A box is associated with transcription factor interactions.
HMGB1 acts as an inflammatory mediator, promoting monocyte migration and cytokine secretion, and facilitates T cell–dendritic cell interaction.
It signals through receptors such as RAGE, TLR2, and TLR4.
HMGB1 is highly conserved and ubiquitous in nuclei and cytoplasm of nearly all cell types, and is a key mediator of inflammation in both sterile and infection-associated responses.
Elevated HMGB1 levels are reported during tissue injury, sepsis, arthritis, and ischemia-reperfusion injury.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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