Thermo Fisher Scientific HMGB1 Polyclonal Antibody
Thermo Fisher Scientific의 HMGB1 Polyclonal Antibody는 다양한 종에서 반응하며, Western blot, IHC, ICC/IF, ELISA 등 다중 응용에 적합합니다. 항원 친화 크로마토그래피로 정제된 고품질 항체로, HMGB1 단백질 연구에 이상적입니다. 연구용으로만 사용됩니다.
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 0.5–1 µg/mL | View 1 publication |
| Immunohistochemistry (IHC) | – | View 3 publications |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100–1:250 | View 3 publications |
| Immunocytochemistry (ICC/IF) | 0.05 µg/mL | View 3 publications |
| Flow Cytometry (Flow) | 1:100 | – |
| ELISA | Assay-dependent | – |
| ChIP assay (ChIP) | 5 µg/mL | – |
| Functional Assay | – | View 1 publication |
| Miscellaneous PubMed | – | View 1 publication |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Bovine, Dog, Human, Mouse, Sheep, Rabbit, Rat |
| Published Species | Dog, Human, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide between amino acids 100–200 of the human HMGB1 protein |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS |
| Contains | 0.02% sodium azide |
| Storage Conditions | 4°C, do not freeze |
| Shipping Conditions | Wet ice |
| RRID | AB_2248274 |
Product Specific Information
This antibody is expected to cross-react with porcine, equine, and rabbit samples based on 100% sequence homology.
Suggested positive control: HeLa whole cell extract, antigen standard for HMGB1 (transient overexpression lysate).
Target Information
HMGB1 (High-mobility group box-1) is a nuclear non-histone DNA-binding chromosomal protein. It can be released from damaged or necrotic cells and acts as a proinflammatory cytokine.
Mouse HMGB1 is a 215 amino acid single-chain polypeptide containing three domains:
- Two DNA-binding domains (HMG box A: aa 9–79; box B: aa 89–162)
- A negatively charged C-terminal acidic tail (aa 186–215)
Residues 28–44 and 180–185 contain nuclear localization signals (NLS).
The B box domain mediates cytokine activity, while the A box is associated with transcription factor interactions.
HMGB1 acts as an inflammatory mediator, promoting monocyte migration and cytokine secretion, and facilitates T cell–dendritic cell interaction.
It signals through receptors such as RAGE, TLR2, and TLR4.
HMGB1 is highly conserved and ubiquitous in nuclei and cytoplasm of nearly all cell types, and is a key mediator of inflammation in both sterile and infection-associated responses.
Elevated HMGB1 levels are reported during tissue injury, sepsis, arthritis, and ischemia-reperfusion injury.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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