Thermo Fisher Scientific H3 K36M oncohistone mutant Recombinant Rabbit Monoclonal Antibody (RM193), ChIP-Verified
H3 K36M 변이체에 특이적으로 반응하는 ChIP 검증된 재조합 토끼 단클론 항체. Western blot, IHC, ICC, ELISA, ChIP 등 다양한 응용에 적합. 높은 특이성과 재현성, 로트 간 일관성 제공. 사람 시료에 반응하며 -20°C에서 보관.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:200–1:1,000 |
| Immunohistochemistry (Paraffin) (IHC-P) | 1:100–1:500 |
| Immunocytochemistry (ICC/IF) | 1:100–1:500 |
| ELISA | 1:250–1:2,500 |
| ChIP assay (ChIP) | 1–5 µg/mL |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | RM193 |
| Immunogen | Peptide corresponding to Acetyl-Histone H3 (Arg36) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.25 mg/mL |
| Purification | Protein A |
| Storage Buffer | PBS (pH 7.2–7.4) with 50% glycerol, 1% BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2661910 |
Additional Formats
Product Specific Information
This antibody specifically reacts to the Histone H3 K36M mutant and shows no cross-reactivity with wild-type Histone H3.
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. These systems clone specific antibody DNA sequences from immunoreactive rabbits, and individual clones are screened to select optimal candidates for production.
Advantages of recombinant rabbit monoclonal antibodies:
- Higher specificity and sensitivity
- Lot-to-lot consistency
- Animal origin-free formulations
- Broader immunoreactivity due to the rabbit immune repertoire
Click here for Master Lot linking information (PDF)
Target Information
Histone H3 is a DNA-binding protein found in the chromatin of all eukaryotic cells. Together with other core histones, it binds DNA to form nucleosomes, playing a central role in transcription regulation, DNA repair, replication, and chromosomal stability.
The N-terminal tail of histone H3 undergoes post-translational modifications such as methylation, acetylation, and phosphorylation, influencing chromatin structure and gene expression.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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